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Epac1 Antibody, FITC Conjugated

Epac1 Antibody, FITC Conjugated size: 0.1ml 333

Supplier BIOSS PRIMARY CONJUGATED ANTIBODIES 1
Price 333
Size 0.1ml
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
Long name FITC Conjugated, Epac1 Polyclonal Antibody
CategoryConjugated Primary Antibodies
ConjugationAnti-FITC Antibody
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenEpac1
SpecificityThis is a highly specific antibody against Epac1
ModificationUnmodified
Modification siteNone
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
SourceKLH conjugated synthetic peptide derived from human Epac1
Gene ID number1019
Tested applicationsIF(IHC-P)
Recommended dilutionsIF(IHC-P)(1:50-200)
Crossreactivity Mouse, Rat, Human
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background Both proteins are multidomain proteins containing an autoinhibitory cAMP-binding domain that inhibits the catalytic region and a DEP domain (dishevelled, EPAC1 mRNA is broadly expressed, EPAC2 has an additional cAMP-binding site in its N-terminus that binds cAMP with low affinity, Egl-10 and pleckstrin homology domain) targeting the membrane anchors, Epac 1 and Epac 2 also interact with light chain 2 (LC2) or MAP1A that serves as a scaffolding structure to stabilize the signal transduction complex, Epac1 and Epac 2, The Epac 1-selective were generated against unique antigenic sequences form near N-terminus and between RasGEFN and Ras GEF domains, The Epac signaling therefore represents a novel mechanism for cAMP signaling with in the cAMP cascade, The to Epac 1are affinity purified over immobilized antigen based chromatography, There are 2 members of the Epac family, kidney and certain brain regions, ovary, whereas expression of EPAC2 mRNA appears to be restricted to the brain and adrenal glands, with particularly high levels occurring in the thyroid, The activation of RaP1 by cAMP is independent of PKA and is mediated by recently discovered family of guanine nucleotide exchange factors (GEFs) called cAMP-GEFs or Epacs
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation Emission494nm/518nm
Synonyms CAMP GEFI, CAMPGEFI, CGEF 1, CGEF1, EPA1, EPAC, EPAC1, Epac 1, Exchange factor directly activated by cAMP 1, Exchange protein directly activated by cAMP 1, MGC21410, RAP guanine nucleotide exchange factor, RAP guanine nucleotide exchange factor 3, RAPGEF3, Rap guanine nucleotide exchange factor GEF 3, Rap1 guanine nucleotide exchange factor directly activated by cAMP, cAMP regulated guanine nucleotide exchange factor I, bcm910
Properties Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targetEpac1 Conjugated
Short name FITC Conjugated, Epac1 Antibody
Technique Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelFITC
Alternative name fluorecein coupled, Epac1 (Antibody to)
Alternative techniqueantibodies

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