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Ephrin B (Tyr317) Antibody, FITC Conjugated

Ephrin B (Tyr317) Antibody, FITC Conjugated size: 0.1ml 350

Price 350
Size 0.1ml
Long name FITC Conjugated, Ephrin B (Tyr317) Polyclonal Antibody
CategoryConjugated Primary Antibodies
ConjugationAnti-FITC Antibody
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenEphrin B Tyr317
SpecificityThis is a highly specific antibody against Ephrin B Tyr317
ModificationPhosphorylation
Modification siteTyr317
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
SourceKLH conjugated synthetic phosphopeptide derived from human Ephrin B around the phosphorylation site of Tyr317
Gene ID number1947
Tested applicationsIF(IHC-P)
Recommended dilutionsIF(IHC-P)(1:50-200)
Crossreactivity Mouse, Rat, Human
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background Ephrin B molecules expressed at the membrane surface bind to the Ephrin B family receptors on target cells during cell to cell contact, Importantly, Recent work demonstrated that Tyr331 of Ephrin B was phosphorylated in HEK293 cells after stimulation by the soluble Ephrin B2 receptor tyrosine kinase, This interaction leads to cell signaling in the target cell but also generates a reverse signal in the cell expressing Ephrin B on its surface, This reverse signaling event is thought to be critical for vessel maturation and neuronal development, tyrosine phosphorylation of Ephrin B is thought to be a critical component of this reverse signaling event, Ephrin B proteins are thought to play key roles in cellular functions as diverse as neuronal migration and blood vessel development
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation Emission494nm/518nm
Synonyms CEK5 ligand, EFNB1, EFNB2_HUMAN, ELK L, ELK ligand, ELKL, EPH related receptor tyrosine kinase ligand 2, Ephrin B Precursor, Ephrin B1, Ephrin B2, Ephrin-B, Ephrin-B2 Tyr317, EphrinB, LERK 2, LERK2, p-Ephrin B2 phospho Y317, Ephrin B2 phospho Y317
Properties Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targetEphrin B (Tyr317) Conjugated
Short name FITC Conjugated, Ephrin B (Tyr317) Antibody
Technique Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelFITC
Alternative name fluorecein coupled, Ephrin B (Tyr317) (Antibody to)
Alternative techniqueantibodies

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