Name : Ephrin B (Tyr324 + Tyr329) Antibody, FITC Conjugated
Supplier : BIOSS PRIMARY CONJUGATED ANTIBODIES 1
Price :350
SKU : GEN1892095953
| Long name | FITC Conjugated, Ephrin B (Tyr324 + Tyr329) Antibody |
| Category | Conjugated Primary Antibodies |
| Conjugation | Anti-FITC Antibody |
| Host Organism | Rabbit (Oryctolagus cuniculus) |
| Target Antigen | Ephrin B Tyr324 + Tyr329 |
| Specificity | These phosphorylation sites are homologous to Tyr323 + Tyr328 in Mouse, This is a highly specific antibody against Ephrin B Tyr324 + Tyr329 |
| Modification | Phosphorylation |
| Modification site | Tyr324 + Tyr329 |
| Clonality | Polyclonal |
| Clone | Polyclonal antibody |
| Concentration | 1ug per 1ul |
| Immunogen_range | 300-346/346 |
| Subcellular location | Cell membrane, Cytoplasm |
| Source | KLH conjugated synthetic phosphopeptide derived from human Ephrin B around the phosphorylation site of Tyr324/329 |
| Gene ID number | 1947 |
| Swiss Prot | P98172 |
| Tested applications | IF(IHC-P) |
| Recommended dilutions | IF(IHC-P)(1:50-200) |
| Crossreactivity | Mouse, Human |
| Crossreactive species details | not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background | Binds promiscuously Eph receptors residing on adjacent cells, Binds to, Binds to, Binds to the receptor tyrosine kinases EPHB3 (preferred), Cell surface transmembrane ligand for Eph receptors, EPHB1 and EPHA1, May play a role in constraining the orientation of longitudinally projecting axons (By similarity), May play a role in constraining the orientation of longitudinally projecting axons (By similarity), The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling, a family of receptor tyrosine kinases which are crucial for migration, and induce the collapse of, and induce the collapse of, commissural axons/growth cones in vitro, commissural axons/growth cones in vitro, leading to contact-dependent bidirectional signaling into neighboring cells, repulsion and adhesion during neuronal, vascular and epithelial development, Binds to the receptor tyrosine kinases EPHB1 and EPHA1 |
| Purification method | This antibody was purified via Protein A |
| Storage conditions | 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission | 494nm/518nm |
| Synonyms | CFNS, EFB1, EFL-3, EFL3, EFNB1, ELK ligand, EPH-related receptor tyrosine kinase ligand 2, EPLG2, Elk-L, Ephrin-B1, LERK-2, LERK2, CFND |
| Properties | Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target | Ephrin B (Tyr324 + Tyr329) Conjugated |
| Short name | FITC Conjugated, Ephrin B (Tyr324 + Tyr329) Antibody |
| Technique | Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label | FITC |
| Alternative name | fluorecein coupled, Ephrin B (Tyr324 and Tyr329) (Antibody to) |
| Alternative technique | antibodies |