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EPS15 Antibody, Biotin Conjugated

EPS15 Antibody, Biotin Conjugated size: 0.1ml 333

Supplier BIOSS PRIMARY CONJUGATED ANTIBODIES 1
Price 333
Size 0.1ml
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
Long name Biotin Conjugated, EPS15 Polyclonal Antibody
CategoryConjugated Primary Antibodies
ConjugationBiotinylated
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenEPS15
SpecificityThis is a highly specific antibody against EPS15
ModificationUnmodified
Modification siteNone
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
SourceKLH conjugated synthetic peptide derived from human EPS15
Tested applicationsIHC-P
Recommended dilutionsIHC-P(1:100-500)
Crossreactivity Mouse, Rat, Human
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background Eps15 is capable of binding the amino terminal portion of Crk via a conserved proline-rich domain, Eps8 and Eps15 are proteins, Eps8 has been shown to associate with the EGF receptor despite its lack of a functional SH2 domain, Further characterization suggests the protein has both a PH domain and a SH3 domain, Miscues in RTK signaling pathways can result in cellular transformation and ultimately in cancer, Overexpression of Eps15 in NIH/3T3 cells causes cellular transformation, Overexpression of Eps8 in both fibroblasts and hematopoietic cells results in an increased mitogenic response to EGF, Two novel EGF receptor substrates designated EGF-receptor pathway substrates 8 and 15, characteristic of all Crk binding proteins (5), have been described, implying involvement in the regulation of cell proliferation, or Eps8 and Eps15, respectively that become tyrosine phosphorylated subsequent to EGF stimulation, the functional significance of which are not yet known, Elucidation of the mechanism by which receptor tyrosine kinases (RTKs) modulate cellular physiology in response to stimuli is critical to the understanding of growth regulation
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation EmissionN/A
Synonyms AF-1P MLLT5
Properties Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targetEPS15 Conjugated
Short name Conjugated, EPS15 Antibody
Technique antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelBiotin
Alternative name biotinilated coupled, EPS15 (Antibody to)
Alternative techniqueantibodies
Identity 3419
Gene EPS15
Long gene name epidermal growth factor receptor pathway substrate 15
Locus 1p32, 3
Discovery year 1994-12-19
GenBank acession BC054006
Entrez gene record 2060
Pubmed identfication 8183552
RefSeq identity NM_001981
Classification EF-hand domain containing
Havana BLAST/BLAT OTTHUMG00000008192

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