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EPS15 Antibody, FITC Conjugated

EPS15 Antibody, FITC Conjugated size: 0.1ml 333

Supplier BIOSS PRIMARY CONJUGATED ANTIBODIES 1
Price 333
Size 0.1ml
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
Long name FITC Conjugated, EPS15 Polyclonal Antibody
CategoryConjugated Primary Antibodies
ConjugationAnti-FITC Antibody
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenEPS15
SpecificityThis is a highly specific antibody against EPS15
ModificationUnmodified
Modification siteNone
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
SourceKLH conjugated synthetic peptide derived from human EPS15
Tested applicationsIF(IHC-P)
Recommended dilutionsIF(IHC-P)(1:50-200)
Crossreactivity Mouse, Rat, Human
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background Eps15 is capable of binding the amino terminal portion of Crk via a conserved proline-rich domain, Eps8 and Eps15 are proteins, Eps8 has been shown to associate with the EGF receptor despite its lack of a functional SH2 domain, Further characterization suggests the protein has both a PH domain and a SH3 domain, Miscues in RTK signaling pathways can result in cellular transformation and ultimately in cancer, Overexpression of Eps15 in NIH/3T3 cells causes cellular transformation, Overexpression of Eps8 in both fibroblasts and hematopoietic cells results in an increased mitogenic response to EGF, Two novel EGF receptor substrates designated EGF-receptor pathway substrates 8 and 15, characteristic of all Crk binding proteins (5), have been described, implying involvement in the regulation of cell proliferation, or Eps8 and Eps15, respectively that become tyrosine phosphorylated subsequent to EGF stimulation, the functional significance of which are not yet known, Elucidation of the mechanism by which receptor tyrosine kinases (RTKs) modulate cellular physiology in response to stimuli is critical to the understanding of growth regulation
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation Emission494nm/518nm
Synonyms AF-1P MLLT5
Properties Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targetEPS15 Conjugated
Short name FITC Conjugated, EPS15 Antibody
Technique Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelFITC
Alternative name fluorecein coupled, EPS15 (Antibody to)
Alternative techniqueantibodies
Identity 3419
Gene EPS15
Long gene name epidermal growth factor receptor pathway substrate 15
Locus 1p32, 3
Discovery year 1994-12-19
GenBank acession BC054006
Entrez gene record 2060
Pubmed identfication 8183552
RefSeq identity NM_001981
Classification EF-hand domain containing
Havana BLAST/BLAT OTTHUMG00000008192

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