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ERAS/HRAS2 Antibody, FITC Conjugated

ERAS/HRAS2 Antibody, FITC Conjugated size: 0.1ml 333

Price 333
Size 0.1ml
Long name FITC Conjugated, ERAS/HRAS2 Polyclonal Antibody
CategoryConjugated Primary Antibodies
ConjugationAnti-FITC Antibody
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenERAS/HRAS2
SpecificityThis is a highly specific antibody against ERAS/HRAS2
ModificationUnmodified
Modification siteNone
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
SourceKLH conjugated synthetic peptide derived from human ERAS/HRAS2
Gene ID number3266
Tested applicationsIF(IHC-P)
Recommended dilutionsIF(IHC-P)(1:50-200)
Crossreactivity Mouse, Rat, Human
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background ERas-null ES cells maintain pluripotency but show significantly reduced growth and tumorigenicity, Ras family, Ras proteins bind GDP/GTP and possess intrinsic GTPase activity, The ERas gene is expressed in embryonic stem (ES) cells and promotes their in vitro proliferation and tumorigenicity, which are rescued by expression of ERas complementary DNA or by activated phosphatidylinositol-3-OH kinase, ERAS belongs to the small GTPase superfamily
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation Emission494nm/518nm
Properties Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targetERAS/HRAS2 Conjugated
Short name FITC Conjugated, ERAS/HRAS2 Antibody
Technique Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelFITC
Alternative name fluorecein coupled, ERAS/HRAS2 (Antibody to)
Alternative techniqueantibodies
Identity 5174
Gene ERAS
Long gene name ES cell expressed Ras
Synonyms gene HRAS2 HRASP
Synonyms gene name v-Ha-ras Harvey rat sarcoma viral oncogene homolog pseudogene
Locus Xp11, 23
Discovery year 2001-06-22
GenBank acession X00419
Entrez gene record 3266
Pubmed identfication 12774123
RefSeq identity NM_181532
Classification RAS type GTPase family
Havana BLAST/BLAT OTTHUMG00000059533

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