| Category | Antibody |
| Concentration | 2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form | Antigen affinity purified |
| Conjugation | Unconjugated |
| Clone | Polyclonal antibody |
| Recognised antigen | ERBB3 (HER3) |
| Host animal | Rabbit (Oryctolagus cuniculus) |
| Clonality | Polyclonal (rabbit origin) |
| Species reactivity | Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications | WB |
| Recommended dilutions | 5-1ug/ml, Western blot: 0 |
| Notes | Titration of the ERBB3 antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting amounts |
| Intented use | This ERBB3 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot # | P21860 |
| Purity | Antigen affinity |
| Description | By in situ hybridization, Carraway et al, EGFR and ERBB2 became markedly more promiscuous as the threshold was lowered, Engelman et al, Kraus et al, Most notably, This gene encodes a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases, This membrane-bound protein has a neuregulin binding domain but not an active kinase domain, a pathway though to be specific to EGFR/ERBB family receptors, also known as HER3, is an enzyme that in humans is encoded by the ERBB3 gene, whereas ERBB3 did not, (1989) mapped the gene to chromosome 12q13, (1994) demonstrated that ERBB3 is a receptor for Heregulin and is capable of mediating HGL-stimulated tyrosine phosphorylation of itself, (2007) found that amplification of MET caused gefitinib resistance by driving ERBB3-dependent activation of phosphoinositide 3-kinase, V-erb-b2 avian erythroblastic leukemia viral oncogene homolog 3 |
| Immunogen | An amino acid sequence from the N-terminus of human ERBB3/HER3 (DWRDIVRDRDAEIVVKDNGR) was used as the immunogen for this ERBB3 antibody |
| Storage | Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the ERBB3 antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties | C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target | ERBB3 (HER3) |
| Short name | ERBB3 Antibody (HER3) |
| Technique | antibodies against human proteins, antibodies for, Antibody |
| Alternative name | v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian) (Antibody to) (HER3) |
| Alternative technique | antibodies |
| Alternative to gene target | ERBB3 and IDBG-39824 and ENSG00000065361 and 2065, ERBB3 and IDBG-636302 and ENSBTAG00000010444 and 785655, Erbb3 and IDBG-197943 and ENSMUSG00000018166 and 13867, Extracellular, c-erbB-3 and c-erbB3 and ErbB-3 and erbB3-S and HER3 and LCCS2 and MDA-BF-1 and p180-ErbB3 and p45-sErbB3 and p85-sErbB3, this GO :0003197 and endocardial cushion development and biological process this GO :0004672 and protein kinase activity and molecular function this GO :0004713 and protein tyrosine kinase activity and molecular function this GO :0004714 and transmembrane receptor protein tyrosine kinase activity and molecular function this GO :0004716 and receptor signaling protein tyrosine kinase activity and molecular function this GO :0004888 and transmembrane signaling receptor activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005524 and ATP binding and molecular function this GO :0005615 and extracellular space and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0005887 and integral component of plasma membrane and cellular component this GO :0006468 and protein phosphorylation and biological process this GO :0007162 and negative regulation of cell adhesion and biological process this GO :0007165 and signal transduction and biological process this GO :0007169 and transmembrane receptor protein tyrosine kinase signaling pathway and biological process this GO :0007173 and epidermal growth factor receptor signaling pathway and biological process this GO :0007422 and peripheral nervous system development and biological process this GO :0007507 and heart development and biological process this GO :0008543 and fibroblast growth factor receptor signaling pathway and biological process this GO :0009968 and negative regulation of signal transduction and biological process this GO :0014037 and Schwann cell differentiation and biological process this GO :0014065 and phosphatidylinositol 3-kinase signaling and biological process this GO :0014068 and positive regulation of phosphatidylinositol 3-kinase signaling and biological process this GO :0016020 and membrane and cellular component this GO :0016021 and integral component of membrane and cellular component this GO :0016323 and basolateral plasma membrane and cellular component this GO :0016324 and apical plasma membrane and cellular component this GO :0016328 and lateral plasma membrane and cellular component this GO :0016772 and transferase activity, this GO :0004672: protein kinase activity, this GO :0004672: protein kinase activity and also this GO :0004713: protein tyrosine kinase activity and also this GO :0004714: transmembrane receptor protein tyrosine kinase activity and also this GO :0004716: receptor signaling protein tyrosine kinase activity and also this GO :0004888: transmembrane signaling receptor activity and also this GO :0005515: protein binding and also this GO :0005524: ATP binding and also this GO :0016772: transferase activity, this GO :0004713: protein tyrosine kinase activity, this GO :0004714: transmembrane receptor protein tyrosine kinase activity, this GO :0004716: receptor signaling protein tyrosine kinase activity, this GO :0004888: transmembrane signaling receptor activity, this GO :0005515: protein binding, this GO :0005524: ATP binding, this GO :0016772: transferase activity, this GO :0019838: growth factor binding, this GO :0030296: protein tyrosine kinase activator activity, this GO :0042803: protein homodimerization activity, this GO :0046982: protein heterodimerization activity, transferase activity, transferring phosphorus-containing groups, transferring phosphorus-containing groups and also this GO :0019838: growth factor binding and also this GO :0030296: protein tyrosine kinase activator activity and also this GO :0042803: protein homodimerization activity and also this GO :0046982: protein heterodimerization activity, transferring phosphorus-containing groups and molecular function this GO :0018108 and peptidyl-tyrosine phosphorylation and biological process this GO :0019838 and growth factor binding and molecular function this GO :0021545 and cranial nerve development and biological process this GO :0030296 and protein tyrosine kinase activator activity and molecular function this GO :0038095 and Fc-epsilon receptor signaling pathway and biological process this GO :0042060 and wound healing and biological process this GO :0042127 and regulation of cell proliferation and biological process this GO :0042803 and protein homodimerization activity and molecular function this GO :0043235 and receptor complex and cellular component this GO :0043524 and negative regulation of neuron apoptotic process and biological process this GO :0045087 and innate immune response and biological process this GO :0046982 and protein heterodimerization activity and molecular function this GO :0048011 and neurotrophin TRK receptor signaling pathway and biological process this GO :0048015 and phosphatidylinositol-mediated signaling and biological process this GO :0051048 and negative regulation of secretion and biological process this GO :0051402 and neuron apoptotic process and biological process this GO :0061098 and positive regulation of protein tyrosine kinase activity and biological process this GO :0097192 and extrinsic apoptotic signaling pathway in absence of ligand and biological process, v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian) |
| Identity | 3431 |
| Gene | ERBB3 |
| Long gene name | erb-b2 receptor tyrosine kinase 3 |
| Synonyms gene | LCCS2 |
| Synonyms gene name | lethal congenital contracture syndrome 2 v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 3 |
| Synonyms | HER3 |
| Synonyms name | human epidermal growth factor receptor 3 |
| Locus | 12q13, 2 |
| Discovery year | 1990-07-15 |
| GenBank acession | M34309 |
| Entrez gene record | 2065 |
| RefSeq identity | NM_001982 |
| Classification | Erb-b2 receptor tyrosine kinases |
| Havana BLAST/BLAT | OTTHUMG00000170140 |
| Locus Specific Databases | LRG_996 |
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