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EYA2 Antibody, FITC Conjugated

EYA2 Antibody, FITC Conjugated size: 0.1ml 333

Supplier BIOSS PRIMARY CONJUGATED ANTIBODIES 1
Price 333
Size 0.1ml
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
Long name FITC Conjugated, EYA2 Polyclonal Antibody
CategoryConjugated Primary Antibodies
ConjugationAnti-FITC Antibody
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenEYA2
SpecificityThis is a highly specific antibody against EYA2
ModificationUnmodified
Modification siteNone
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
SourceKLH conjugated synthetic peptide derived from human EYA2
Gene ID number2139
Tested applicationsIF(IHC-P)
Recommended dilutionsIF(IHC-P)(1:50-200)
Crossreactivity Mouse, Rat, Human
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background A 271 amino acid domain at the carboxy-terminal is highly conserved amongst the members of the eyes absent family, EYA2, EYA2 carboxy-terminal interacts with the G Alpha z and G Alphai 2 proteins, EYA2 is expressed relatively late in development in the cytoplasm of extensor tendons and ligaments of the phalangeal elements of the limb, EYA2 is one of four members of the eyes absent family, EYA2 is translocated to the nucleus by Six proteins, Pax3 induces the expression of Eya2 in a cascade that is necessary and sufficient for myogenesis, This interaction prevents Six proteins from translocating EYA2 to the nucleus, acts as a transcriptional activator in connective tissue patterning through its PST domain, and the developing eye, branchial arches, central nervous system, cranial placodes, like EYA1, which functions as a transactivation domain, which interact through their domain and homeodomain with EYA2, while the PST (proline-serive-threonin)-rich amino-terminal is highly divergent, 1 encodes Eya2 (eyes absent), A gene on chromosome 20q13
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation Emission494nm/518nm
Synonyms EAB1
Properties Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targetEYA2 Conjugated
Short name FITC Conjugated, EYA2 Antibody
Technique Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelFITC
Alternative name fluorecein coupled, eyes absent homolog 2 (Drosophila) (Antibody to)
Alternative techniqueantibodies
Alternative to gene target DNA-templated and biological process this GO :0006355 and regulation of transcription, DNA-templated and biological process this GO :0006470 and protein dephosphorylation and biological process this GO :0007275 and multicellular organismal development and biological process this GO :0007501 and mesodermal cell fate specification and biological process this GO :0014706 and striated muscle tissue development and biological process this GO :0016576 and histone dephosphorylation and biological process this GO :0035335 and peptidyl-tyrosine dephosphorylation and biological process this GO :0097192 and extrinsic apoptotic signaling pathway in absence of ligand and biological process this GO :0097345 and mitochondrial outer membrane permeabilization and biological process, EAB1, EYA2 and IDBG-643097 and ENSBTAG00000013336 and 615264, EYA2 and IDBG-79457 and ENSG00000064655 and 2139, Eya2 and IDBG-212752 and ENSMUSG00000017897 and 14049, nuclei, protein binding, this GO :0000287 and magnesium ion binding and molecular function this GO :0004725 and protein tyrosine phosphatase activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005813 and centrosome and cellular component this GO :0006281 and DNA repair and biological process this GO :0006351 and transcription, this GO :0000287: magnesium ion binding, this GO :0000287: magnesium ion binding and also this GO :0004725: protein tyrosine phosphatase activity and also this GO :0005515: protein binding, this GO :0004725: protein tyrosine phosphatase activity, this GO :0005515: protein binding, eyes absent homolog 2 (Drosophila)
Identity 3520
Gene EYA2
Long gene name EYA transcriptional coactivator and phosphatase 2
Synonyms gene name eyes absent (Drosophila) homolog 2 eyes absent homolog 2 (Drosophila)
Locus 20q13, 12
Discovery year 1997-06-24
Entrez gene record 2139
Pubmed identfication 9020840
RefSeq identity NM_005244
Classification EYA transcriptional coactivator and phosphatases
Havana BLAST/BLAT OTTHUMG00000033041

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