Name : FEN1 Antibody, Biotin Conjugated
Supplier : BIOSS PRIMARY CONJUGATED ANTIBODIES 1
Price :333
SKU : GEN5860641012
| Long name | Biotin Conjugated, FEN1 Polyclonal Antibody |
| Category | Conjugated Primary Antibodies |
| Conjugation | Biotinylated |
| Host Organism | Rabbit (Oryctolagus cuniculus) |
| Target Antigen | FEN1 |
| Specificity | This is a highly specific antibody against FEN1 |
| Modification | Unmodified |
| Modification site | None |
| Clonality | Polyclonal |
| Clone | Polyclonal antibody |
| Concentration | 1ug per 1ul |
| Source | KLH conjugated synthetic peptide derived from human FEN1 |
| Gene ID number | 2237 |
| Tested applications | IHC-P, WB |
| Recommended dilutions | IHC-P(1:100-500), WB(1:100-1000) |
| Crossreactivity | Mouse, Rat, Human |
| Crossreactive species details | not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background | Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions, Also involved in replication and repair of rDNA and in repairing mitochondrial DNA, Also involved in the long patch base excision repair (LP-BER) pathway, Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, During DNA replication, It enters the flap from the 5'-end and then tracks to cleave the flap base, and exhibits RNase H activity, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment, leaving a nick for ligation, Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair |
| Purification method | This antibody was purified via Protein A |
| Storage conditions | 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission | N/A |
| Synonyms | FEN-1 MF1 |
| Properties | Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target | FEN1 Conjugated |
| Short name | Conjugated, FEN1 Antibody |
| Technique | antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label | Biotin |
| Alternative name | biotinilated coupled, FEN1 (Antibody to) |
| Alternative technique | antibodies |
| Identity | 3650 |
| Gene | FEN1 |
| Long gene name | flap structure-specific endonuclease 1 |
| Synonyms gene | RAD2 |
| Synonyms name | maturation factor-1 DNase IV |
| Locus | 11q12, 2 |
| Discovery year | 1995-02-03 |
| GenBank acession | L37374 |
| Entrez gene record | 2237 |
| Pubmed identfication | 7774922 |
| RefSeq identity | NM_004111 |
| Havana BLAST/BLAT | OTTHUMG00000168162 |