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GAD65 Antibody, FITC Conjugated

GAD65 Antibody, FITC Conjugated size: 0.1ml 333

Price 333
Size 0.1ml
Long name FITC Conjugated, GAD65 Polyclonal Antibody
CategoryConjugated Primary Antibodies
ConjugationAnti-FITC Antibody
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenGAD65
SpecificityThis is a highly specific antibody against GAD65
ModificationUnmodified
Modification siteNone
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
Immunogen_range541-567/585
Subcellular locationCytoplasm
SourceKLH conjugated synthetic peptide derived from human GAD65
Gene ID number2572
Swiss ProtQ05329
Tested applicationsIF(IHC-P)
Recommended dilutionsIF(IHC-P)(1:50-200)
Crossreactivity Mouse, Rat, Human
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes, Alternative splicing results in multiple transcript variants that encode the same protein, Oct 2008], The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid, This gene may also play a role in the stiff man syndrome, [provided by RefSeq, identified as a major autoantigen in insulin-dependent diabetes, This gene encodes one of several forms of glutamic acid decarboxylase
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation Emission494nm/518nm
Synonyms GAD65
Properties Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targetGAD65 Conjugated
Short name FITC Conjugated, GAD65 Antibody
Technique Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelFITC
Alternative name fluorecein coupled, GAD65 (Antibody to)
Alternative techniqueantibodies
Identity 4093
Gene GAD2
Long gene name glutamate decarboxylase 2
Synonyms gene name 65kD) glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa) , glutamate decarboxylase 2 (pancreatic islets and brain
Locus 10p12, 1
Discovery year 1991-11-29
GenBank acession AJ251501
Entrez gene record 2572
Pubmed identfication 2039509
RefSeq identity NM_000818
Havana BLAST/BLAT OTTHUMG00000017836

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