| Long name | GADD153/DDIT3 Primary Polyclonal Antibody |
| Also known as | GADD153/DDIT3 PAb |
| Category | Primary Antibodies |
| Conjugation | Unconjugated |
| Target Antigen | GADD153/DDIT3 |
| Specificity | This is a highly specific antibody against GADD153/DDIT3 |
| Modification(s) | None |
| Modification site(s) | Unmodified antibody |
| Clonality | Polyclonal antibody |
| Clone number | Polyclonal antibody |
| Concentration | 1ug per 1ul |
| Immunogen range | 96-120/168 |
| Source | This antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human DDIT3 |
| Gene ID number | 1649 |
| Swiss Prot | P35638 |
| Tested Applications | IF(IHC-P), IHC-P |
| Recommended dilutions | IF(IHC-P)(1:50-200), IHC-P(1:100-500) |
| Cross reactivity | Mouse, Rat, Human |
| Cross reactive species details | not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Background of the target antigen | ATF4-dependent transcriptional activation of asparagine synthetase (ASNS), Acts as a dominant-negative regulator of C/EBP-induced transcription: dimerizes with members of the C/EBP family, BBC3/PUMA, BCL2L11/BIM and ERO1L, CEBPA-dependent transcriptional activation of hepcidin (HAMP) and CEBPB-mediated expression of peroxisome proliferator-activated receptor gamma (PPARG), IL23, IL6, IL8, Inhibits the canonical Wnt signaling pathway by binding to TCF7L2/TCF4, Negatively regulates, PPP1R15A/GADD34, Plays a dual role both as an inhibitor of CCAAT/enhancer-binding protein (C/EBP) function and as an activator of other genes, Plays a regulatory role in the inflammatory response through the induction of caspase-11 (CASP4/CASP11) which induces the activation of caspase-1 (CASP1) and both these caspases increase the activation of pro-IL1B to mature IL1B which is involved in the inflammatory response, Plays an essential role in the response to a wide variety of cell stresses and induces cell cycle arrest and apoptosis in response to ER stress, Positively regulates the transcription of TRIB3, TNFRSF1B/DR5, and inhibits the expression of C/EBP regulated genes, expression of BCL2 and MYOD1, impairing its DNA-binding properties and repressing its transcriptional activity, impairs their association with C/EBP binding sites in the promoter regions, Multifunctional transcription factor in ER stress response |
| Purification method | This antibody was purified via Protein A |
| Storage conditions | 50% glycerol and 0, Store at -20°, 09% sodium azide, C for up to 1 year, Keep the antibody in aqueous buffered solution containing 1% BSA |
| Synonym names | C/EBP zeta, C/EBP-homologous protein, C/EBP-homologous protein 1, CCAAT/enhancer-binding protein homologous protein, CEBPZ, CHOP-1, CHOP1, DDIT-3, DDIT3, DNA damage-inducible transcript 3 protein, GADD153, Growth arrest and DNA damage-inducible protein GADD153, CHOP |
| Properties | C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24° |
| Gene target | GADD153/DDIT3 |
| Short name | GADD153/DDIT3 Antibody |
| Technique | antibodies against human proteins, antibodies for, Antibody |
| Alternative name | GADD153/Desoxyribonucleic acid-damage-inducible transcript 3 (Antibody to) |
| Alternative technique | antibodies |
| Alternative to gene target | CEBPZ and CHOP and CHOP-10 and CHOP10 and GADD153, DDIT3 and IDBG-42805 and ENSG00000175197 and 1649, DDIT3 and IDBG-635159 and ENSBTAG00000031544 and 777788, DNA-templated and biological process this GO :0006915 and apoptotic process and biological process this GO :0006974 and cellular response to DNA damage stimulus and biological process this GO :0006983 and ER overload response and biological process this GO :0006986 and response to unfolded protein and biological process this GO :0006987 and activation of signaling protein activity involved in unfolded protein response and biological process this GO :0007050 and cell cycle arrest and biological process this GO :0008134 and transcription factor binding and molecular function this GO :0016055 and Wnt signaling pathway and biological process this GO :0030968 and endoplasmic reticulum unfolded protein response and biological process this GO :0032757 and positive regulation of interleukin-8 production and biological process this GO :0034976 and response to endoplasmic reticulum stress and biological process this GO :0042594 and response to starvation and biological process this GO :0042789 and mRNA transcription from RNA polymerase II promoter and biological process this GO :0043161 and proteasome-mediated ubiquitin-dependent protein catabolic process and biological process this GO :0043433 and negative regulation of sequence-specific DNA binding transcription factor activity and biological process this GO :0043525 and positive regulation of neuron apoptotic process and biological process this GO :0043565 and sequence-specific DNA binding and molecular function this GO :0043620 and regulation of DNA-dependent transcription in response to stress and biological process this GO :0044267 and cellular protein metabolic process and biological process this GO :0044324 and regulation of transcription involved in anterior/posterior axis specification and biological process this GO :0045087 and innate immune response and biological process this GO :0045454 and cell redox homeostasis and biological process this GO :0045662 and negative regulation of myoblast differentiation and biological process this GO :0045892 and negative regulation of transcription, DNA-templated and biological process this GO :0045893 and positive regulation of transcription, DNA-templated and biological process this GO :0045944 and positive regulation of transcription from RNA polymerase II promoter and biological process this GO :0051209 and release of sequestered calcium ion into cytosol and biological process this GO :0070059 and intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress and biological process this GO :0090090 and negative regulation of canonical Wnt signaling pathway and biological process this GO :2000016 and negative regulation of determination of dorsal identity and biological process, Ddit3 and IDBG-194880 and ENSMUSG00000025408 and 13198, nuclei, sequence-specific DNA binding, this GO :0001955 and blood vessel maturation and biological process this GO :0003677 and DNA binding and molecular function this GO :0003700 and sequence-specific DNA binding transcription factor activity and molecular function this GO :0003714 and transcription corepressor activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005654 and nucleoplasm and cellular component this GO :0005770 and late endosome and cellular component this GO :0006355 and regulation of transcription, this GO :0003677: DNA binding, this GO :0003677: DNA binding and also this GO :0003700: sequence-specific DNA binding transcription factor activity and also this GO :0003714: transcription corepressor activity and also this GO :0005515: protein binding and also this GO :0008134: transcription factor binding and also this GO :0043565: sequence-specific DNA binding, this GO :0003700: sequence-specific DNA binding transcription factor activity, this GO :0003714: transcription corepressor activity, this GO :0005515: protein binding, this GO :0008134: transcription factor binding, this GO :0043565: sequence-specific DNA binding, DNA-damage-inducible transcript 3 |
| Identity | 2726 |
| Gene | DDIT3 |
| Long gene name | DNA damage inducible transcript 3 |
| Synonyms gene name | DNA-damage-inducible transcript 3 |
| Synonyms | CHOP10 GADD153 CHOP |
| Synonyms name | C/EBP zeta |
| Locus | 12q13, 3 |
| Discovery year | 1991-09-11 |
| GenBank acession | BC003637 |
| Entrez gene record | 1649 |
| Pubmed identfication | 1990262 |
| RefSeq identity | NM_004083 |
| Havana BLAST/BLAT | OTTHUMG00000170046 |
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