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GAPDH Polyconal Antibody, Biotin Conjugated

GAPDH Polyconal Antibody, Biotin Conjugated size: 0.1ml 333

Supplier BIOSS PRIMARY CONJUGATED ANTIBODIES 1
Price 333
Size 0.1ml
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
Long name Biotin Conjugated, GAPDH Polyconal Antibody
CategoryConjugated Primary Antibodies
ConjugationBiotinylated
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenGAPDH
SpecificityThis is a highly specific antibody against GAPDH
ModificationUnmodified
Modification siteNone
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
SourceRecombinant full length human GAPDH
Gene ID number2597
Swiss ProtP04406
Tested applicationsWB
Recommended dilutionsWB(1:100-1000)
Crossreactivity Mouse, Rat, Human
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes, DNA replication and apoptosis, Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity), Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate, HDAC2 and PRKDC, Modulates the organization and assembly of the cytoskeleton, Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, Participates in nuclear events including transcription, RNA transport, Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation, respectively, thereby playing a role in glycolysis and nuclear functions, Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation EmissionN/A
Properties Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targetGAPDH Polyconal Conjugated
Short name Conjugated, GAPDH Polyconal Antibody
Technique antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelBiotin
Alternative name biotinilated coupled, GAPDH Polyconal (Antibody to)
Alternative techniqueantibodies
Identity 4141
Gene GAPDH
Long gene name glyceraldehyde-3-phosphate dehydrogenase
Synonyms gene GAPD
Locus 12p13, 31
Discovery year 2001-06-22
GenBank acession AF261085
Entrez gene record 2597
Pubmed identfication 3170585
RefSeq identity NM_002046
Havana BLAST/BLAT OTTHUMG00000137379

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