Filters

GATA4 (Ser105) Antibody, Biotin Conjugated

GATA4 (Ser105) Antibody, Biotin Conjugated size: 0.1ml 350

Supplier BIOSS PRIMARY CONJUGATED ANTIBODIES 1
Price 350
Size 0.1ml
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
Long name Biotin Conjugated, GATA4 (Ser105) Polyclonal Antibody
CategoryConjugated Primary Antibodies
ConjugationBiotinylated
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenGATA4 Ser105
SpecificityThis is a highly specific antibody against GATA4 Ser105
ModificationPhosphorylation
Modification siteSer105
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
SourceKLH conjugated synthetic phosphopeptide derived from human GATA4 around the phosphorylation site of Ser105
Gene ID number2626
Tested applications IHC-P, WB
Recommended dilutions IHC-P(1:100-500), WB(1:100-1000)
Crossreactivity Mouse, Rat, Human
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background AGATCTTA, Although expression patterns of the various GATA transcription factors may overlap, Both GATA-4 and GATA-6 are found in heart, GATA-1 is erythroid-specific and is responsible for the regulated transcription of erythroid genes, GATA-2 is expressed in embryonic brain and liver, GATA-3 is highly expressed in T lymphocytes, GATA-4, GATA-5 and GATA-6 comprise a subfamily of transcription factors, GATA-5 expression has been observed in differentiated heart and gut tissues and is present throughout the course of development in the heart, HeLa and endothelial cells, It is an essential component in the generation of the erythroid lineage, Studies with a modified GATA consensus sequence, This indicates broader regulatory capabilities of GATA-2 and GATA-3 than GATA-1, as well as in erythroid cells, but not GATA-4 expression, have shown that GATA-2 and GATA-3 recognize this mutated consensus while GATA-1 has poor recognition of this sequence, it is not yet apparent how the GATA factors are able to discriminate in binding their appropriate target sites, lung and liver tissues exhibit GATA-6, pancreas and ovary, Members of the GATA family share a conserved zinc finger DNA-binding domain and are capable of binding the WGATAR consensus sequence
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation EmissionN/A
Properties Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targetGATA4 (Ser105) Conjugated
Short name Conjugated, GATA4 (Ser105) Antibody
Technique antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelBiotin
Alternative name biotinilated coupled, GATA4 (Ser105) (Antibody to)
Alternative techniqueantibodies
Identity 4173
Gene GATA4
Long gene name GATA binding protein 4
Synonyms gene name GATA-binding protein 4
Locus 8p23, 1
Discovery year 1994-11-30
GenBank acession AK097060
Entrez gene record 2626
Pubmed identfication 7665171
RefSeq identity NM_002052
Classification GATA zinc finger domain containing
Havana BLAST/BLAT OTTHUMG00000090800

Similar products

Subscribe to our Newsletter