| Long name | GCLC Primary Polyclonal Antibody |
| Also known as | GCLC PAb |
| Category | Primary Antibodies |
| Conjugation | Unconjugated |
| Target Antigen | GCLC |
| Specificity | This is a highly specific antibody against GCLC |
| Modification(s) | None |
| Modification site(s) | Unmodified antibody |
| Clonality | Polyclonal antibody |
| Clone number | Polyclonal antibody |
| Concentration | 1ug per 1ul |
| Source | This antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human GCLC |
| Gene ID number | 2730 |
| Tested Applications | IF(IHC-P), IHC-P, WB |
| Recommended dilutions | IF(IHC-P)(1:50-200), IHC-P(1:100-500), WB(1:100-1000) |
| Cross reactivity | Mouse, Rat, Human |
| Cross reactive species details | not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Background of the target antigen | A His370Leu amino acid change in g-GCSc causes deficiencies in activity which are responsible for hemolytic anemia and low red blood cell glutathione levels, Cadium regulates transcription through proteins Met-4, Cbf1, Chemopreventive compounds cause increased levels of g-GCSc in kidney tissues, H2O2 regulation depends on the Yap1 protein and the presence of glutamate, Met-31 and Met-32, Oxidants, a DNA binding protein, also designated g-L-glutamate-L-cysteine ligase catalytic subunit (GLCLC), brain, cadium and methyl mercury upregulate the transcription of g-GCS, g-GCS, g-GCS is the first enzyme involved and the rate determining step in glutathione biosynthesis, g-GCSc associates with a regulatory or modifier subunit, g-GCSc is expressed in hemocytes, g-GCSm (g-glutamylcysteine synthetase light subunit), glutamine and lysine, inhibits transcription of g-GCS, liver and kidney, to form a heterodimer, which may protect against chemically induced carcinogenesis, Defects in GCLC are the cause of hemolytic anemia, The GCLC gene consists of 16 exons and encodes the 636 amino acid protein g-GCSc (g-glutamylcysteine synthetase heavy subunit) |
| Purification method | This antibody was purified via Protein A |
| Storage conditions | 50% glycerol and 0, Store at -20°, 09% sodium azide, C for up to 1 year, Keep the antibody in aqueous buffered solution containing 1% BSA |
| Synonym names | GCL, GCLC, GCLM, GCS, GCS Heavy Chain antibody GLCL, GLCLC, GLCLR, GSH1_HUMAN, Gamma GCS, Gamma glutamylcysteine synthetase, Gamma-ECS, Gamma-glutamylcysteine synthetase, Glutamate cysteine ligase catalytic subunit, Glutamate cysteine ligase catalytic subunit, Glutamate cysteine ligase gamma glutamylcysteine synthetase regulatory 30, Glutamate cysteine ligase modier subunit, Glutamate cysteine ligase regulatory protein, Glutamate cysteine ligase regulatory subunit, Glutamate--cysteine ligase catalytic subunit, 8kD, Gamma ECS |
| Properties | C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24° |
| Gene target | GCLC |
| Short name | GCLC Antibody |
| Technique | antibodies against human proteins, antibodies for, Antibody |
| Alternative name | catalytic subunit (Antibody to), glutamate-cysteine ligase |
| Alternative technique | antibodies |
| Alternative to gene target | Cytoplasm, DNA-templated and biological process this GO :0046685 and response to arsenic-containing substance and biological process this GO :0046982 and protein heterodimerization activity and molecular function this GO :0050662 and coenzyme binding and molecular function this GO :0050880 and regulation of blood vessel size and biological process this GO :0051409 and response to nitrosative stress and biological process this GO :0051900 and regulation of mitochondrial depolarization and biological process this GO :1901687 and glutathione derivative biosynthetic process and biological process this GO :2001237 and negative regulation of extrinsic apoptotic signaling pathway and biological process, GCL and GCS and GLCL and GLCLC, GCLC and IDBG-628748 and ENSBTAG00000015571 and 512468, GCLC and IDBG-91041 and ENSG00000001084 and 102724876, Gclc and IDBG-186028 and ENSMUSG00000032350 and 14629, catalytic subunit, coenzyme binding, this GO :0000096 and sulfur amino acid metabolic process and biological process this GO :0000287 and magnesium ion binding and molecular function this GO :0004357 and glutamate-cysteine ligase activity and molecular function this GO :0005524 and ATP binding and molecular function this GO :0005737 and cytoplasm and cellular component this GO :0005829 and cytosol and cellular component this GO :0006534 and cysteine metabolic process and biological process this GO :0006536 and glutamate metabolic process and biological process this GO :0006749 and glutathione metabolic process and biological process this GO :0006750 and glutathione biosynthetic process and biological process this GO :0006805 and xenobiotic metabolic process and biological process this GO :0006979 and response to oxidative stress and biological process this GO :0008637 and apoptotic mitochondrial changes and biological process this GO :0009408 and response to heat and biological process this GO :0009410 and response to xenobiotic stimulus and biological process this GO :0009725 and response to hormone and biological process this GO :0016595 and glutamate binding and molecular function this GO :0017109 and glutamate-cysteine ligase complex and cellular component this GO :0019852 and L-ascorbic acid metabolic process and biological process this GO :0031397 and negative regulation of protein ubiquitination and biological process this GO :0032436 and positive regulation of proteasomal ubiquitin-dependent protein catabolic process and biological process this GO :0034641 and cellular nitrogen compound metabolic process and biological process this GO :0043066 and negative regulation of apoptotic process and biological process this GO :0043524 and negative regulation of neuron apoptotic process and biological process this GO :0043531 and ADP binding and molecular function this GO :0044281 and small molecule metabolic process and biological process this GO :0045454 and cell redox homeostasis and biological process this GO :0045892 and negative regulation of transcription, this GO :0000287: magnesium ion binding, this GO :0000287: magnesium ion binding and also this GO :0004357: glutamate-cysteine ligase activity and also this GO :0005524: ATP binding and also this GO :0016595: glutamate binding and also this GO :0043531: ADP binding and also this GO :0046982: protein heterodimerization activity and also this GO :0050662: coenzyme binding, this GO :0004357: glutamate-cysteine ligase activity, this GO :0005524: ATP binding, this GO :0016595: glutamate binding, this GO :0043531: ADP binding, this GO :0046982: protein heterodimerization activity, this GO :0050662: coenzyme binding, 2729, glutamate-cysteine ligase |
| Identity | 4311 |
| Gene | GCLC |
| Long gene name | glutamate-cysteine ligase catalytic subunit |
| Synonyms gene | GLCLC GLCL |
| Synonyms gene name | catalytic subunit , glutamate-cysteine ligase |
| Synonyms | GCS |
| Locus | 6p12, 1 |
| Discovery year | 1993-11-24 |
| GenBank acession | M90656 |
| Entrez gene record | 2729 |
| Pubmed identfication | 1350904 |
| Havana BLAST/BLAT | OTTHUMG00000160220 |
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