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GCLC Antibody, ALEXA FLUOR 350

GCLC Antibody, ALEXA FLUOR 350 size: 100ul 332

Supplier BIOSS PRIMARY CONJUGATED ANTIBODIES
Price 332
Size 100ul
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
Long name ALEXA FLUOR 350 Conjugated, GCLC Polyclonal Antibody
Also known asAnti-GCLC PAb ALEXA FLUOR 350
CategoryConjugated Primary Antibodies
Conjugated with 350, ALEXA FLUOR®
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenGCLC
SpecificityThis is a highly specific antibody against GCLC
ModificationUnmodified
Modification SiteNone
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
SourceThis antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human GCLC
Gene ID Number2730
Tested applicationsIF(IHC-P)
Recommended dilutionsIF(IHC-P)(1:50-200)
Crossreactivity Mouse, Rat, Human
Cross-reactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Background of the antigen A His370Leu amino acid change in g-GCSc causes deficiencies in activity which are responsible for hemolytic anemia and low red blood cell glutathione levels, Cadium regulates transcription through proteins Met-4, Cbf1, Chemopreventive compounds cause increased levels of g-GCSc in kidney tissues, H2O2 regulation depends on the Yap1 protein and the presence of glutamate, Met-31 and Met-32, Oxidants, a DNA binding protein, also designated g-L-glutamate-L-cysteine ligase catalytic subunit (GLCLC), brain, cadium and methyl mercury upregulate the transcription of g-GCS, g-GCS, g-GCS is the first enzyme involved and the rate determining step in glutathione biosynthesis, g-GCSc associates with a regulatory or modifier subunit, g-GCSc is expressed in hemocytes, g-GCSm (g-glutamylcysteine synthetase light subunit), glutamine and lysine, inhibits transcription of g-GCS, liver and kidney, to form a heterodimer, which may protect against chemically induced carcinogenesis, Defects in GCLC are the cause of hemolytic anemia, The GCLC gene consists of 16 exons and encodes the 636 amino acid protein g-GCSc (g-glutamylcysteine synthetase heavy subunit)
PurificationPurified by Protein A
Storage conditions 50% glycerol and 0, Keep refrigerated at 2 to 8 degrees Celcius for up to one year, 09% sodium azide, Store this antibody in aqueous buffered solution containing 1% BSA
Excitation emission343nm/442nm
Synonyms GCS
Properties ALEXA FLUOR they should be stored frozen at - 24°, Alexa Fluor 350 conjugates can be used in multi-color flow cytometry with FACS's equipped with a second red laser or red diode, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies, C, C for long term storage and for short term at + 5°, For facs or microscopy Alexa 1 conjugate
Conjugation ALEXA FLUOR 350, Alexa Fluor
Gene targetGCLC
Short nameGCLC Antibody
Technique antibodies against human proteins, antibodies for, Antibody
LabelALEXA FLUOR 350
Alternative name ALEXA FLUOR 350, catalytic subunit (Antibody to), glutamate-cysteine ligase
Alternative techniqueantibodies
Alternative to gene target Cytoplasm, DNA-templated and biological process this GO :0046685 and response to arsenic-containing substance and biological process this GO :0046982 and protein heterodimerization activity and molecular function this GO :0050662 and coenzyme binding and molecular function this GO :0050880 and regulation of blood vessel size and biological process this GO :0051409 and response to nitrosative stress and biological process this GO :0051900 and regulation of mitochondrial depolarization and biological process this GO :1901687 and glutathione derivative biosynthetic process and biological process this GO :2001237 and negative regulation of extrinsic apoptotic signaling pathway and biological process, GCL and GCS and GLCL and GLCLC, GCLC and IDBG-628748 and ENSBTAG00000015571 and 512468, GCLC and IDBG-91041 and ENSG00000001084 and 102724876, Gclc and IDBG-186028 and ENSMUSG00000032350 and 14629, catalytic subunit, coenzyme binding, this GO :0000096 and sulfur amino acid metabolic process and biological process this GO :0000287 and magnesium ion binding and molecular function this GO :0004357 and glutamate-cysteine ligase activity and molecular function this GO :0005524 and ATP binding and molecular function this GO :0005737 and cytoplasm and cellular component this GO :0005829 and cytosol and cellular component this GO :0006534 and cysteine metabolic process and biological process this GO :0006536 and glutamate metabolic process and biological process this GO :0006749 and glutathione metabolic process and biological process this GO :0006750 and glutathione biosynthetic process and biological process this GO :0006805 and xenobiotic metabolic process and biological process this GO :0006979 and response to oxidative stress and biological process this GO :0008637 and apoptotic mitochondrial changes and biological process this GO :0009408 and response to heat and biological process this GO :0009410 and response to xenobiotic stimulus and biological process this GO :0009725 and response to hormone and biological process this GO :0016595 and glutamate binding and molecular function this GO :0017109 and glutamate-cysteine ligase complex and cellular component this GO :0019852 and L-ascorbic acid metabolic process and biological process this GO :0031397 and negative regulation of protein ubiquitination and biological process this GO :0032436 and positive regulation of proteasomal ubiquitin-dependent protein catabolic process and biological process this GO :0034641 and cellular nitrogen compound metabolic process and biological process this GO :0043066 and negative regulation of apoptotic process and biological process this GO :0043524 and negative regulation of neuron apoptotic process and biological process this GO :0043531 and ADP binding and molecular function this GO :0044281 and small molecule metabolic process and biological process this GO :0045454 and cell redox homeostasis and biological process this GO :0045892 and negative regulation of transcription, this GO :0000287: magnesium ion binding, this GO :0000287: magnesium ion binding and also this GO :0004357: glutamate-cysteine ligase activity and also this GO :0005524: ATP binding and also this GO :0016595: glutamate binding and also this GO :0043531: ADP binding and also this GO :0046982: protein heterodimerization activity and also this GO :0050662: coenzyme binding, this GO :0004357: glutamate-cysteine ligase activity, this GO :0005524: ATP binding, this GO :0016595: glutamate binding, this GO :0043531: ADP binding, this GO :0046982: protein heterodimerization activity, this GO :0050662: coenzyme binding, 2729, glutamate-cysteine ligase
Identity 4311
Gene GCLC
Long gene name glutamate-cysteine ligase catalytic subunit
Synonyms gene GLCLC GLCL
Synonyms gene name catalytic subunit , glutamate-cysteine ligase
Locus 6p12, 1
Discovery year 1993-11-24
GenBank acession M90656
Entrez gene record 2729
Pubmed identfication 1350904
Havana BLAST/BLAT OTTHUMG00000160220

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