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GPR77 Antibody, FITC Conjugated

GPR77 Antibody, FITC Conjugated size: 0.1ml 333

Supplier BIOSS PRIMARY CONJUGATED ANTIBODIES 1
Price 333
Size 0.1ml
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
Long name FITC Conjugated, GPR77 Polyclonal Antibody
CategoryConjugated Primary Antibodies
ConjugationAnti-FITC Antibody
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenGPR77
SpecificityThis is a highly specific antibody against GPR77
ModificationUnmodified
Modification siteNone
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
Subcellular locationExtracellular
SourceKLH conjugated synthetic peptide derived from human GPF77
Gene ID number27202
Tested applications IF(IHC-P), FCM
Recommended dilutions IF(IHC-P)(1:50-200), FCM(1:20-100)
CrossreactivityHuman
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background C5L2 is an integral membrane protein that belongs to the Gprotein-coupled receptor 1 family, C5L2 weakly couples to Gi-mediated signaling pathways and is widely expressed in the hippocampus, It is coexpressed with the C5a receptor (C5AR) on polymorphonuclear neutrophils and may modulate the activity of C5AR, Research suggests that C5L2 may be required for the in vitro release of high mobility group box 1 protein (HMGB1), The function of the C5a receptors, a chemotactic and inflammatory peptide, also designated G protein-coupled receptor 77, frontal cortex and liver, hypothalamus, is a receptor for C5a anaphylatoxin chemotactic receptor (C5L2), is still unclear, particularly that of C5L2, pons, Anaphylatoxin
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation Emission494nm/518nm
Synonyms C5L2
Properties Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targetGPR77 Conjugated
Short name FITC Conjugated, GPR77 Antibody
Technique Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelFITC
Alternative name fluorecein coupled, G protein-coupled receptor 77 (Antibody to)
Alternative techniqueantibodies
Alternative to gene target GPR77 and IDBG-59470 and ENSG00000134830 and 27202, GPR77 and IDBG-639987 and ENSBTAG00000037735 and 522144, Gpr77 and IDBG-146283 and ENSMUSG00000074361 and 319430, Plasma membranes, protein binding, this GO :0004930 and G-protein coupled receptor activity and molecular function this GO :0004944 and C5a anaphylatoxin receptor activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005886 and plasma membrane and cellular component this GO :0006935 and chemotaxis and biological process this GO :0006954 and inflammatory response and biological process this GO :0007186 and G-protein coupled receptor signaling pathway and biological process this GO :0009925 and basal plasma membrane and cellular component this GO :0016021 and integral component of membrane and cellular component this GO :0032720 and negative regulation of tumor necrosis factor production and biological process this GO :0045087 and innate immune response and biological process this GO :0045177 and apical part of cell and cellular component this GO :0050679 and positive regulation of epithelial cell proliferation and biological process this GO :0070374 and positive regulation of ERK1 and ERK2 cascade and biological process this GO :0090024 and negative regulation of neutrophil chemotaxis and biological process this GO :1900165 and negative regulation of interleukin-6 secretion and biological process this GO :2000482 and regulation of interleukin-8 secretion and biological process, this GO :0004930: G-protein coupled receptor activity, this GO :0004930: G-protein coupled receptor activity and also this GO :0004944: C5a anaphylatoxin receptor activity and also this GO :0005515: protein binding, this GO :0004944: C5a anaphylatoxin receptor activity, this GO :0005515: protein binding, G protein-coupled receptor 77
Identity 4527
Gene C5AR2
Long gene name complement component 5a receptor 2
Synonyms gene GPR77
Synonyms gene name G protein-coupled receptor 77
Locus 19q13, 32
Discovery year 2000-01-20
GenBank acession AB038237
Entrez gene record 27202
Pubmed identfication 11165367
RefSeq identity NM_018485
Classification Complement component GPCRs

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