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GPX4 Antibody, Biotin Conjugated

GPX4 Antibody, Biotin Conjugated size: 0.1ml 333

Price 333
Size 0.1ml
Long name Biotin Conjugated, GPX4 Polyclonal Antibody
CategoryConjugated Primary Antibodies
ConjugationBiotinylated
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenGPX4
SpecificityThis is a highly specific antibody against GPX4
ModificationUnmodified
Modification siteNone
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
SourceKLH conjugated synthetic peptide derived from human Glutathione Peroxidase 4
Gene ID number2879
Tested applications IHC-P, WB
Recommended dilutions IHC-P(1:100-500), WB(1:100-1000)
Crossreactivity Mouse, Rat, Human
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background All mammalian GPx family members, Extracellular plasma GPx (pGPx or GPx3) is mainly expressed by the kidney from where it is released into the blood circulation, GPx2, Phospholipid hydroperoxide GPx4 (PH-GPx) expressed in most tissues, The classical cytosolic/mitochondrial GPx1 (cGPx) is a selenium-dependent enzyme, There are several isoforms which differ in their primary structure and localization, also known as gastrointestinal GPx (GI-GPx), can reduce many hydroperoxides including hydroperoxides integrated in membranes, except for the recently described Cys containing GPx3 and epididymis-specific secretory GPx (eGPx or GPx5) isoforms, first of the GPx family to be discovered, hydroperoxy lipids in low density lipoprotein or thymine, is an intracellular enzyme expressed only at the epithelium of the gastrointestinal tract, possess selenocysteine at the active site, Glutathione peroxidases (Gpxs) are ubiquitously expressed proteins which catalyze the reduction of hydrogen peroxides and organic hydroperoxides by glutathione
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation EmissionN/A
Synonyms PHGPx MCSP
Properties Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targetGPX4 Conjugated
Short name Conjugated, GPX4 Antibody
Technique antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelBiotin
Alternative name biotinilated coupled, GPX4 (Antibody to)
Alternative techniqueantibodies
Identity 4556
Gene GPX4
Long gene name glutathione peroxidase 4
Synonyms gene name glutathione peroxidase 4 (phospholipid hydroperoxidase)
Synonyms name phospholipid hydroperoxidase
Locus 19p13, 3
Discovery year 1993-05-03
GenBank acession BC039849 X71973
Entrez gene record 2879
Pubmed identfication 8287691 8039723 10464096
RefSeq identity NM_002085
Classification Selenoproteins
Havana BLAST/BLAT OTTHUMG00000181874

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