Filters

GRAP Antibody, FITC Conjugated

GRAP Antibody, FITC Conjugated size: 0.1ml 333

Supplier BIOSS PRIMARY CONJUGATED ANTIBODIES 1
Price 333
Size 0.1ml
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
Long name FITC Conjugated, GRAP Polyclonal Antibody
CategoryConjugated Primary Antibodies
ConjugationAnti-FITC Antibody
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenGRAP
SpecificityThis is a highly specific antibody against GRAP
ModificationUnmodified
Modification siteNone
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
SourceKLH conjugated synthetic peptide derived from human GRAP
Tested applicationsIF(IHC-P)
Recommended dilutionsIF(IHC-P)(1:50-200)
Crossreactivity Mouse, Rat, Human
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background For instance, GRB10 (also referred to as GRB-IR), GRB14 and Grap (for GRB2-related adapter protein), GRB7, Grap also associates with the Ras guanine nucleotide exchange factor Sos 1 via its amino terminal SH3 domain, Grap shares the highest degree of homology with GRB2 exhibiting 59% sequence identity with GRB2, Members include GRB2, Signaling by such receptors involves a series of intermediates characterized by SH2 domains that bind tyrosine phosphorylated receptors by a direct interaction between the SH2 domain and specific receptor sequences, The Grap SH2 domain is capable of binding to the activated stem cell factor receptor, While GRB10 and GRB14 are most closely related to GRB7, c-Kit and the erythropoietin receptor (EpoR), the GRB family of proteins lack a defined catalytic activity and are entirely composed of SH2 and SH3 domains, Many growth factors function by binding receptors with intrinsic tyrosine kinase activity
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation Emission494nm/518nm
Properties Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targetGRAP Conjugated
Short name FITC Conjugated, GRAP Antibody
Technique Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelFITC
Alternative name fluorecein coupled, GRAP (Antibody to)
Alternative techniqueantibodies
Identity 4562
Gene GRAP
Long gene name GRB2-related adaptor protein
Locus 17p11, 2
Discovery year 1998-09-29
GenBank acession U52518
Entrez gene record 10750
Pubmed identfication 8647802 8995379
RefSeq identity NM_006613
Classification SH2 domain containing
Havana BLAST/BLAT OTTHUMG00000059436

Similar products

Subscribe to our Newsletter