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GRB10/14 Antibody, Biotin Conjugated

GRB10/14 Antibody, Biotin Conjugated size: 0.1ml 333

Supplier BIOSS PRIMARY CONJUGATED ANTIBODIES 1
Price 333
Size 0.1ml
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
Long name Biotin Conjugated, GRB10/14 Polyclonal Antibody
CategoryConjugated Primary Antibodies
ConjugationBiotinylated
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenGRB10/14
SpecificityThis is a highly specific antibody against GRB10/14
ModificationUnmodified
Modification siteNone
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
Immunogen_range574-594/594
Subcellular locationCytoplasm
SourceKLH conjugated synthetic peptide derived from human GRB10
Gene ID number2887, 2888
Tested applications IHC-P, WB
Recommended dilutions IHC-P(1:100-500), WB(1:100-1000)
Crossreactivity Mouse, Rat, Human
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background Binds to, Blocks association between INSR and IRS1 and IRS2 and prevents insulin-stimulated IRS1 and IRS2 tyrosine phosphorylation, Delays and reduces AKT1 phosphorylation in response to insulin stimulation, GRB14 is a potent inhibitor of insulin-stimulated MAPK3 phosphorylation, May inhibit NEDD4-mediated degradation of KDR/VEGFR-2, May play a role in mediating insulin-stimulated ubiquitination of INSR, Negatively regulates Wnt signaling by interacting with LRP6 intracellular portion and interfering with the binding of AXIN1 to LRP6, Plays a critical role regulating PDPK1 membrane translocation in response to insulin stimulation and serves as an adapter protein to recruit PDPK1 to activated insulin receptor, Positive regulator of the KDR/VEGFR-2 signaling pathway, Recruits NEDD4 to IGF1R, The inhibitory effect can be achieved by 2 mechanisms: interference with the signaling pathway and increased receptor degradation, activated receptors tyrosine kinases, and suppress signals from, including the insulin (INSR) and insulin-like growth factor (IGF1R) receptors, increased internalization and degradation by both the proteasomal and lysosomal pathways, leading to IGF1R ubiquitination, leading to proteasomal degradation, thus promoting PKB/AKT1 phosphorylation and transduction of the insulin signal, Adapter protein which modulates coupling of a number of cell surface receptor kinases with specific signaling pathways
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation EmissionN/A
Properties Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targetGRB10/14 Conjugated
Short name Conjugated, GRB10/14 Antibody
Technique antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelBiotin
Alternative name biotinilated coupled, GRB10/14 (Antibody to)
Alternative techniqueantibodies
Identity 4564
Gene GRB10
Long gene name growth factor receptor bound protein 10
Synonyms gene name growth factor receptor-bound protein 10
Locus 7p12, 1
Discovery year 1996-05-30
Entrez gene record 2887
RefSeq identity NM_005311
Classification Pleckstrin homology domain containing SH2 domain containing
Havana BLAST/BLAT OTTHUMG00000150622
Locus Specific Databases LRG_1032

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