| Alternative name 1 | Clone: 4B4, IF/WB/IP (native only), Mouse, Rat, Rat Mab anti-Human, ~70-85kD, Heat Shock Factor 1 (HSF1) |
| Alternative name 2 | Clone: 4B4, IF/WB/IP (native only), Mouse, Rat, Rat Mab antibody-Human, ~70-85kD, Heat Shock Factor 1 (HSF1) |
| Alternative name 3 | Clone: 4B4, IF/WB/IP (native only), Mus musculus, Rattus norvegicus, Rattus norvegicus Mab antibody-Homo sapiens, ~70-85kD, Heat Shock Factor 1 (HSF1) |
| Alternative name 4 | anti-Heat Shock Factor 1 HSF1 ~70-85kD Clone: 4B4 / native only |
| Alternative name 5 | Heat Shock Factor 1 HSF1 ~70-85kD Clone: 4B4 / native only |
| Clonality | Monoclonal Antibody |
| Description | Amongst the advantages of the monoclonal antibodies are: the fact that while the hybridoma takes a bit longer to be produced, If used correctly and according the protocol, in comparison to the polyclonal antibodies monoclonal antibodies are highly specific to a given epitope and can be used in applications where specific targeting is required, once the line is ready there is virtually an endless supply of these antibodie throughout time with little to no variations in recognition sites of the antigen in different batches, this antibody will provide excellent and reproducible results with guaranteed success for the tested and confirmed applications, This is a monoclonal antibody which is greatly purified and with high binding affinity for the antigen that it is risen against |
| Storage and handling | Antibodies in a liquid form can be shipped and stored for a short period of time at +4 degrees Celsius, antibodies that are lyophilized can be transported at ambient temperature and stored for short terms at +4 degrees Celsius, for long term storage (up to one year) 25-50% glycerol or ethylene glycol should be added and then the container has to be stored at -20°, for longer periods - at -20 , C, Generally |
| Advisory | For antibodies in a liquid form or reconstituted lyophilized antibodies small amounts of the soultion could be captured on the cap or the walls of the container, Right before use you could briefly centrifuge the vial to collect all of the solution on the bottom, To keep the quality and the affinity of the antibody cycles of freezing and thawing should be avoided |
| Properties | Depending on the epitopes used human ELISA kits can be cross reactive to many other species, Mainly analyzed are human serum, Modern , cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies, human cell culture supernatants and biological samples, plasma, primarily , saliva, urine, , (Homo sapiens, Homo sapiens sapiens), Human proteins, humans , ssp |
| About | Each murine monoclonal anibody has his own affinity specific for the clone, Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes, Rats are used to make rat monoclonal anti mouse antibodies, Rats , There are less rat- than mouse clones however, Monoclonals of this antigen are available in different clones, Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats, genes from rodents , of the genus |
| Test | A/J, BALB/c, Mouse are mature after 40 days for females and 55 days for males, SCID while the CD-1 is outbred as strain, The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year, Transgenic, congenic and inbread strains are known for C57BL/6, knock-out, Mouse , mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab, or |
| Latin name | Rattus norvegicus, Mus musculus |
| French translation | anticorps |
| Gene target | 4B4, IF/ /IP (native only), Monoclonal , Heat Shock Factor 1 (HSF1) ~70-85kD |
| Short name | Clone: 4B4, IF/ /IP (native only), Mouse Monoclonal antibody- Mouse, Heat Shock Factor 1 (HSF1) ~70-85kD |
| Technique | Monoclonals or monoclonal antibodies, mouses, Mouse |
| Host | Rat |
| Species | Humans, Mouses, Rats, Rat |
| Alternative name | IF/Western Blot/IP (native only), Mouse, Rat, Rat Mouse monoclonal (Antibody to)-H, clonality: 4B4, sapiens, ~70-85kD, Heat Shock Factor 1 (heat shock transcription factor 1) |
| Alternative technique | murine |
| Alternative to gene target | DNA-templated and biological process this GO :0006355 and regulation of transcription, DNA-templated and biological process this GO :0006468 and protein phosphorylation and biological process this GO :0006952 and defense response and biological process this GO :0007143 and female meiotic division and biological process this GO :0007283 and spermatogenesis and biological process this GO :0008285 and negative regulation of cell proliferation and biological process this GO :0009408 and response to heat and biological process this GO :0032496 and response to lipopolysaccharide and biological process this GO :0032720 and negative regulation of tumor necrosis factor production and biological process this GO :0034605 and cellular response to heat and biological process this GO :0040018 and positive regulation of multicellular organism growth and biological process this GO :0043234 and protein complex and cellular component this GO :0043565 and sequence-specific DNA binding and molecular function this GO :0045087 and innate immune response and biological process this GO :0045120 and pronucleus and cellular component this GO :0045944 and positive regulation of transcription from RNA polymerase II promoter and biological process this GO :0060136 and embryonic process involved in female pregnancy and biological process this GO :0090231 and regulation of spindle checkpoint and biological process, HSF1 and IDBG-40498 and ENSG00000185122 and 3297, HSF1 and IDBG-643592 and ENSBTAG00000020751 and 506235, HSTF1, Hsf1 and IDBG-155311 and ENSMUSG00000022556 and 15499, nuclei, sequence-specific DNA binding, this GO :0000122 and negative regulation of transcription from RNA polymerase II promoter and biological process this GO :0000978 and RNA polymerase II core promoter proximal region sequence-specific DNA binding and molecular function this GO :0001078 and RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription and molecular function this GO :0001162 and RNA polymerase II intronic transcription regulatory region sequence-specific DNA binding and molecular function this GO :0001701 and in utero embryonic development and biological process this GO :0001892 and embryonic placenta development and biological process this GO :0003677 and DNA binding and molecular function this GO :0003682 and chromatin binding and molecular function this GO :0003700 and sequence-specific DNA binding transcription factor activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0006351 and transcription, this GO :0000978: RNA polymerase II core promoter proximal region sequence-specific DNA binding, this GO :0000978: RNA polymerase II core promoter proximal region sequence-specific DNA binding and also this GO :0001078: RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription and also this GO :0001162: RNA polymerase II intronic transcription regulatory region sequence-specific DNA binding and also this GO :0003677: DNA binding and also this GO :0003682: chromatin binding and also this GO :0003700: sequence-specific DNA binding transcription factor activity and also this GO :0005515: protein binding and also this GO :0043565: sequence-specific DNA binding, this GO :0001078: RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription, this GO :0001162: RNA polymerase II intronic transcription regulatory region sequence-specific DNA binding, this GO :0003677: DNA binding, this GO :0003682: chromatin binding, this GO :0003700: sequence-specific DNA binding transcription factor activity, this GO :0005515: protein binding, this GO :0043565: sequence-specific DNA binding, heat shock transcription factor 1 |
| Identity | 5224 |
| Gene | HSF1 |
| Long gene name | heat shock transcription factor 1 |
| Synonyms | HSTF1 |
| Locus | 8q24, 3 |
| Discovery year | 1994-09-12 |
| GenBank acession | M64673 |
| Entrez gene record | 3297 |
| Pubmed identfication | 1871105 |
| RefSeq identity | NM_005526 |
| Havana BLAST/BLAT | OTTHUMG00000174604 |
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