| Antigen detectable with this ELISA kit | Heat Shock Transcription Factor 1 (HSF1) |
| Species reactivity | Homo sapiens (Human) |
| Range of detection | 0, 0, 1, 10ng/mL, 2, 5ng/mL, 25ng/mL, 313-20ng/mL The standard curve concentrations used for the ELISA&rsquo, 313ng/mL, 5ng/mL, 625ng/mL, s were 20ng/mL |
| Sensitivity | 115ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application | ELISA |
| Sample types tested for this ELISA kit | cell lysates and other biological fluids, Tissue homogenates |
| Time for performing t hourse test | 4, 5 hoursours |
| Test principle | 10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Heat Shock Transcription Factor 1 (HSF1), The concentration of Heat Shock Transcription Factor 1 (HSF1) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heat Shock Transcription Factor 1 (HSF1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Heat Shock Transcription Factor 1 (HSF1), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling | Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory | Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties | E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test | ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target | Heat Shock Transcription Factor 1 (HSF1) |
| Short name | Heat Shock Transcription Factor 1 (HSF1) ELISA kit |
| Technique | &hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host | antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name | Heat Shock Transcription Factor 1 (heat shock transcription factor 1) Enzyme-linked immunosorbent assay reagent |
| Alternative technique | elisas |
| Alternative to gene target | DNA-templated and biological process this GO :0006355 and regulation of transcription, DNA-templated and biological process this GO :0006468 and protein phosphorylation and biological process this GO :0006952 and defense response and biological process this GO :0007143 and female meiotic division and biological process this GO :0007283 and spermatogenesis and biological process this GO :0008285 and negative regulation of cell proliferation and biological process this GO :0009408 and response to heat and biological process this GO :0032496 and response to lipopolysaccharide and biological process this GO :0032720 and negative regulation of tumor necrosis factor production and biological process this GO :0034605 and cellular response to heat and biological process this GO :0040018 and positive regulation of multicellular organism growth and biological process this GO :0043234 and protein complex and cellular component this GO :0043565 and sequence-specific DNA binding and molecular function this GO :0045087 and innate immune response and biological process this GO :0045120 and pronucleus and cellular component this GO :0045944 and positive regulation of transcription from RNA polymerase II promoter and biological process this GO :0060136 and embryonic process involved in female pregnancy and biological process this GO :0090231 and regulation of spindle checkpoint and biological process, HSF1 and IDBG-40498 and ENSG00000185122 and 3297, HSF1 and IDBG-643592 and ENSBTAG00000020751 and 506235, HSTF1, Hsf1 and IDBG-155311 and ENSMUSG00000022556 and 15499, nuclei, sequence-specific DNA binding, this GO :0000122 and negative regulation of transcription from RNA polymerase II promoter and biological process this GO :0000978 and RNA polymerase II core promoter proximal region sequence-specific DNA binding and molecular function this GO :0001078 and RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription and molecular function this GO :0001162 and RNA polymerase II intronic transcription regulatory region sequence-specific DNA binding and molecular function this GO :0001701 and in utero embryonic development and biological process this GO :0001892 and embryonic placenta development and biological process this GO :0003677 and DNA binding and molecular function this GO :0003682 and chromatin binding and molecular function this GO :0003700 and sequence-specific DNA binding transcription factor activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0006351 and transcription, this GO :0000978: RNA polymerase II core promoter proximal region sequence-specific DNA binding, this GO :0000978: RNA polymerase II core promoter proximal region sequence-specific DNA binding and also this GO :0001078: RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription and also this GO :0001162: RNA polymerase II intronic transcription regulatory region sequence-specific DNA binding and also this GO :0003677: DNA binding and also this GO :0003682: chromatin binding and also this GO :0003700: sequence-specific DNA binding transcription factor activity and also this GO :0005515: protein binding and also this GO :0043565: sequence-specific DNA binding, this GO :0001078: RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription, this GO :0001162: RNA polymerase II intronic transcription regulatory region sequence-specific DNA binding, this GO :0003677: DNA binding, this GO :0003682: chromatin binding, this GO :0003700: sequence-specific DNA binding transcription factor activity, this GO :0005515: protein binding, this GO :0043565: sequence-specific DNA binding, heat shock transcription factor 1 |
| Identity | 5224 |
| Gene | HSF1 |
| Long gene name | heat shock transcription factor 1 |
| Synonyms | HSTF1 |
| Locus | 8q24, 3 |
| Discovery year | 1994-09-12 |
| GenBank acession | M64673 |
| Entrez gene record | 3297 |
| Pubmed identfication | 1871105 |
| RefSeq identity | NM_005526 |
| Havana BLAST/BLAT | OTTHUMG00000174604 |