Filters

HEXA Antibody

HEXA Antibody size: 0.1mg 389

Supplier NJS POLY
Price 389
Size 0.1mg
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
CategoryAntibody
Concentration2ml sterile DI water, 5mg/ml if reconstituted with 0
FormAntigen affinity purified
ConjugationUnconjugated
ClonePolyclonal antibody
Recognised antigenHEXA
Host animalRabbit (Oryctolagus cuniculus)
ClonalityPolyclonal (rabbit origin)
Species reactivity Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus), Rat , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens)
Tested applications IHC-P, WB
Recommended dilutions5-1ug/ml, 5-1ug/ml, IHC (Paraffin): 0, Western blot: 0
Notes Titration of the HEXA antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting amounts
Intented useThis HEXA antibodyis to be used only for research purposes and not for diagnostics
Uniprot #P06865
PurityAntigen affinity
Description Arg-424, Chimeric constructs were expressed in HeLa cells and selected constructs were produced in the baculovirus expression system to determine their ability to degrade GM2 ganglioside in the presence of GM2 activator protein, Even though the alpha and beta subunits of hexosaminidase A can both cleave GalNAc residues, Hexosaminidase A and the cofactor GM2 activator protein catalyze the degradation of the GM2 gangliosides and other molecules containing terminal N-acetyl hexosamines The HEXA gene encodes the alpha subunit of hexosaminidase A, The alpha subunit contains a key residue, Their results allowed them to define two noncontiguous sequences in the alpha subunit (amino acids 1-191 and 403-529) which, a lysosomal enzyme involved in the breakdown of gangliosides, conferred activity against the sulfated substrate, only the alpha subunit is able to hydrolyze GM2 gangliosides, when substituted into analogous positions in the beta subunit, which is essential for binding the N-acetyl-neuramanic residue of GM2 gangliosides, HEXA (Hexosaminidase A) is an enzyme that in humans is encoded by the HEXA gene
Immunogen alpha subunit (DVMAYNKLNVFHWHLVD) was used as the immunogen for this HEXA antibody, An amino acid sequence from the middle region of human Hexosaminidase A
Storage Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the HEXA antibody can be stored for up to one month at 4oC, After reconstitution
PropertiesC, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°
Gene targetHEXA
Short nameHEXA Antibody
Technique antibodies against human proteins, antibodies for, Antibody
Alternative nameHEXA (Antibody to)
Alternative techniqueantibodies
Identity 4878
Gene HEXA
Long gene name hexosaminidase subunit alpha
Synonyms gene name hexosaminidase A (alpha polypeptide)
Synonyms name Tay Sachs disease GM2 gangliosidosis beta-hexosaminidase subunit alpha
Locus 15q23
Discovery year 2001-06-22
GenBank acession M13520
Entrez gene record 3073
Pubmed identfication 2952641 3013851
RefSeq identity NM_000520
Havana BLAST/BLAT OTTHUMG00000133445
Locus Specific Databases Hexosaminidase A, Tay-Sachs Disease ALSOD, the Amyotrophic Lateral Sclerosis Online Genetic Database

Similar products

Subscribe to our Newsletter