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HIF1 alpha Antibody

HIF1 alpha Antibody size: 0.4 ml 406

Supplier NJS POLY
Price 406
Size 0.4 ml
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
CategoryAntibody
Concentration pH 7, with 0, 09% sodium azide, 4, In 1X PBS
FormPurified
ConjugationUnconjugated
ClonePolyclonal antibody
Recognised antigenHIF1 alpha
Host animalRabbit (Oryctolagus cuniculus)
ClonalityPolyclonal (rabbit origin)
Species reactivity Due to limited knowledge and inability to test the antibody against all known species, Hamster , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens)
Tested applications ELISA, IF, IHC, WB
Recommended dilutionsIHC (Paraffin): 1:10-1:50, Immunofluorescence: 1:10-1:50, Western blot: 1:1000
NotesTitration of the HIF-1 alpha antibody may be required due to differences in protocols and secondary/substrate sensitivity
Intented useThis HIF1 alpha antibodyis to be used only for research purposes and not for diagnostics
Uniprot #Q16665
PurityPurified
Description HIF1 is a heterodimer composed of an alpha subunit and a beta subunit, Overexpression of a natural antisense transcript (aHIF) of this gene has been shown to be associated with nonpapillary renal carcinomas, The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT), This protein encodes the alpha subunit of HIF-1, Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia
ImmunogenA portion of amino acids 1-30 from the human protein was used as the immunogen for this HIF1 alpha antibody
Storage Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, Aliquot the HIF1 alpha antibody and store frozen at -20 deg
PropertiesC, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°
Gene targetHIF1 alpha
Short nameHIF1 alpha Antibody
Technique antibodies against human proteins, antibodies for, Antibody
Alternative nameHIF1 a (Antibody to)
Alternative techniqueantibodies
Identity 4910
Gene HIF1A
Long gene name hypoxia inducible factor 1 alpha subunit
Synonyms gene name alpha subunit (basic helix-loop-helix transcription factor) , hypoxia inducible factor 1
Synonyms MOP1 HIF-1alpha PASD8 HIF1 bHLHe78
Locus 14q23, 2
Discovery year 1995-03-27
GenBank acession U22431
Entrez gene record 3091
Pubmed identfication 8786149 9079689
RefSeq identity NM_001530
Classification Basic helix-loop-helix proteins
Havana BLAST/BLAT OTTHUMG00000140344

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