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HIF2a Antibody

HIF2a Antibody size: 0.1mg 389

Supplier NJS POLY
Price 389
Size 0.1mg
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
CategoryAntibody
Concentration2ml sterile DI water, 5mg/ml if reconstituted with 0
FormAntigen affinity purified
ConjugationUnconjugated
ClonePolyclonal antibody
Recognised antigenHIF2a
Host animalRabbit (Oryctolagus cuniculus)
ClonalityPolyclonal (rabbit origin)
Species reactivity Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus), Rat , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens)
Tested applicationsWB
Recommended dilutions5-1ug/ml, Western blot: 0
Notes Titration of the HIF2a antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting amounts
Intented useThis HIF2a antibodyis to be used only for research purposes and not for diagnostics
Uniprot #Q9JHS1
PurityAntigen affinity
Description Furthermore, HIF2a binds to and activates transcription from the HIF1a response element derived from the 3-prime flanking region of the EPO gene, HIF2a is predominantly expressed in highly vascularized tissues of adult humans and in endothelial cells of the mouse adult and embryo, In addition, It is expressed at relatively higher levels in villus sections of placenta and in lung samples compared with other tissues examined, Like HIF1a, Moreover, The human gene contains 15 exons and spans at least 120 kb, The positions of the introns within the genomic region encoding the N-terminal bHLH-PAS domains of HIF2a and AHR are similar, The predicted mouse protein is 88% identical to human, a bHLH-PAS transcription factor that induces EPO gene expression in cultured cells in response to hypoxia, it may represent an important regulator of vascularization, perhaps involving the regulation of endothelial cell gene expression in response to hypoxia, suggesting that the 5-prime ends of the 2 genes may have arisen from a gene duplication event, the predicted protein shares 48% sequence identity with HIF1a, the variation in HIF-2 alpha influences the relative contribution of aerobic and anaerobic metabolism and hence the maximum sustainable metabolic power for a given event duration, HIF-2 alpha is also designated EPAS1 whose gene is mapped to 2p21-p16
Immunogen mouse and rat), An amino acid sequence from the middle region of human HIF-2 alpha (HALDSENMTKSHQNLCTKGQ) was used as the immunogen for this HIF2a antibody (100% homologous in human
Storage Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the HIF2a antibody can be stored for up to one month at 4oC, After reconstitution
PropertiesC, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°
Gene targetHIF2a
Short nameHIF2a Antibody
Technique antibodies against human proteins, antibodies for, Antibody
Alternative nameHIF2a (Antibody to)
Alternative techniqueantibodies
Identity 3374
Gene EPAS1
Long gene name endothelial PAS domain protein 1
Synonyms MOP2 PASD2 HIF2A HLF bHLHe73
Synonyms name HIF-1 alpha-like factor
Locus 2p21
Discovery year 1998-05-29
GenBank acession U81984
Entrez gene record 2034
Pubmed identfication 9000051 9079689 18378852
RefSeq identity NM_001430
Classification Basic helix-loop-helix proteins
Havana BLAST/BLAT OTTHUMG00000128818

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