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Histone H3 Antibody, Biotin Conjugated

Histone H3 Antibody, Biotin Conjugated size: 0.1ml 333

Price 333
Size 0.1ml
Long name Biotin Conjugated, Histone H3 Polyclonal Antibody
CategoryConjugated Primary Antibodies
ConjugationBiotinylated
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenHistone H3
SpecificityThis is a highly specific antibody against Histone H3
ModificationUnmodified
Modification siteNone
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
Immunogen_range90-136/136
Subcellular locationNucleus
SourceKLH conjugated synthetic peptide derived from human Histone H3
Gene ID number3020
Swiss ProtP84243
Tested applications IHC-P, WB
Recommended dilutions IHC-P(1:100-500), WB(1:100-1000)
Crossreactivity Mouse, Pig, Rat, Human
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis, DNA accessibility is regulated via a complex set of post-translational modifications of histones, DNA repair, DNA replication and chromosomal stability, Deposited at sites of nucleosomal displacement throughout transcribed genes, Histones thereby play a central role in transcription regulation, Nucleosomes wrap and compact DNA into chromatin, also called histone code, and nucleosome remodeling, limiting DNA accessibility to the cellular machineries which require DNA as a template, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin, Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation EmissionN/A
Synonyms H3, H3F3A, Histone H3, PP781, 3, 3A, H3F3
Properties Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targetHistone H3 Conjugated
Short name Conjugated, Histone H3 Antibody
Technique antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelBiotin
Alternative name biotinilated coupled, Histone H3 (Antibody to)
Alternative techniqueantibodies

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