| Category | Antibody |
| Concentration | 2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form | Antigen affinity purified |
| Conjugation | Unconjugated |
| Clone | Polyclonal antibody |
| Recognised antigen | HRG |
| Host animal | Rabbit (Oryctolagus cuniculus) |
| Clonality | Polyclonal (rabbit origin) |
| Species reactivity | Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications | IHC-P, WB |
| Recommended dilutions | 5-1ug/ml, 5-1ug/ml, IHC (Paraffin): 0, Western blot: 0 |
| Notes | Titration of the HRG antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting amounts |
| Intented use | This HRG antibodyis to be used only for research purposes and not for diagnostics |
| Gene ID # | 3273 |
| Purity | Antigen affinity |
| Description | HRG contains two cystatin-like domains and is located in plasma and platelets, HRG is thought to have multiple roles as a protein in the human blood, It also can inhibit rosette formation and interacts with heparin, It is mapped to 3q27, Mutations in this gene lead to thrombophilia due to abnormal histidine-rich glycoprotein levels, The protein can bind heme, Two of the proteins effects, angiogenesis and coagulation, dyes and divalent metal ions, including roles in immunity, indicate a potential prothrombotic effect, the inhibition of fibrinolysis and the reduction of inhibition of coagulation, thrombospondin and plasminogen, 3, Histidine-rich glycoprotein is a protein that in humans is encoded by the HRG gene |
| Immunogen | Human partial recombinant protein (AA 19-200) was used as the immunogen for this HRG antibody |
| Storage | Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the HRG antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties | C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target | HRG |
| Short name | HRG Antibody |
| Technique | antibodies against human proteins, antibodies for, Antibody |
| Alternative name | histidine-rich glycoprotein (Antibody to) |
| Alternative technique | antibodies |
| Alternative to gene target | Extracellular, HPRG and HRGP and THPH11, HRG and IDBG-634671 and ENSBTAG00000001265 and 101909599, HRG and IDBG-69039 and ENSG00000113905 and 3273, Hrg and IDBG-148872 and ENSMUSG00000022877 and 94175, metal ion binding, this GO :0001525 and angiogenesis and biological process this GO :0002576 and platelet degranulation and biological process this GO :0002839 and positive regulation of immune response to tumor cell and biological process this GO :0004867 and serine-type endopeptidase inhibitor activity and molecular function this GO :0004869 and cysteine-type endopeptidase inhibitor activity and molecular function this GO :0005102 and receptor binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005576 and extracellular region and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0006935 and chemotaxis and biological process this GO :0007162 and negative regulation of cell adhesion and biological process this GO :0007596 and blood coagulation and biological process this GO :0008201 and heparin binding and molecular function this GO :0008270 and zinc ion binding and molecular function this GO :0008285 and negative regulation of cell proliferation and biological process this GO :0010468 and regulation of gene expression and biological process this GO :0010543 and regulation of platelet activation and biological process this GO :0010593 and negative regulation of lamellipodium assembly and biological process this GO :0010951 and negative regulation of endopeptidase activity and biological process this GO :0014070 and response to organic cyclic compound and biological process this GO :0015886 and heme transport and biological process this GO :0016525 and negative regulation of angiogenesis and biological process this GO :0019865 and immunoglobulin binding and molecular function this GO :0020037 and heme binding and molecular function this GO :0030168 and platelet activation and biological process this GO :0030193 and regulation of blood coagulation and biological process this GO :0030308 and negative regulation of cell growth and biological process this GO :0031093 and platelet alpha granule lumen and cellular component this GO :0031982 and vesicle and cellular component this GO :0032956 and regulation of actin cytoskeleton organization and biological process this GO :0033629 and negative regulation of cell adhesion mediated by integrin and biological process this GO :0034395 and regulation of transcription from RNA polymerase II promoter in response to iron and biological process this GO :0036019 and endolysosome and cellular component this GO :0042730 and fibrinolysis and biological process this GO :0043065 and positive regulation of apoptotic process and biological process this GO :0043254 and regulation of protein complex assembly and biological process this GO :0043395 and heparan sulfate proteoglycan binding and molecular function this GO :0043537 and negative regulation of blood vessel endothelial cell migration and biological process this GO :0045087 and innate immune response and biological process this GO :0046872 and metal ion binding and molecular function this GO :0050730 and regulation of peptidyl-tyrosine phosphorylation and biological process this GO :0050832 and defense response to fungus and biological process this GO :0051894 and positive regulation of focal adhesion assembly and biological process this GO :0051918 and negative regulation of fibrinolysis and biological process this GO :0061474 and pha this GO lysosome membrane and cellular component this GO :0070062 and extracellular vesicular exosome and cellular component this GO :0072562 and blood microparticle and cellular component this GO :0097037 and heme export and biological process this GO :1900747 and negative regulation of vascular endothelial growth factor signaling pathway and biological process this GO :2000504 and positive regulation of blood vessel remodeling and biological process this GO :2001027 and negative regulation of endothelial cell chemotaxis and biological process, this GO :0004867: serine-type endopeptidase inhibitor activity, this GO :0004867: serine-type endopeptidase inhibitor activity and also this GO :0004869: cysteine-type endopeptidase inhibitor activity and also this GO :0005102: receptor binding and also this GO :0005515: protein binding and also this GO :0008201: heparin binding and also this GO :0008270: zinc ion binding and also this GO :0019865: immunoglobulin binding and also this GO :0020037: heme binding and also this GO :0043395: heparan sulfate proteoglycan binding and also this GO :0046872: metal ion binding, this GO :0004869: cysteine-type endopeptidase inhibitor activity, this GO :0005102: receptor binding, this GO :0005515: protein binding, this GO :0008201: heparin binding, this GO :0008270: zinc ion binding, this GO :0019865: immunoglobulin binding, this GO :0020037: heme binding, this GO :0043395: heparan sulfate proteoglycan binding, this GO :0046872: metal ion binding, 280820, histidine-rich glycoprotein |
| Identity | 5181 |
| Gene | HRG |
| Long gene name | histidine rich glycoprotein |
| Synonyms gene name | histidine-rich glycoprotein |
| Synonyms | HRGP HPRG |
| Synonyms name | histidine-proline rich glycoprotein thrombophilia due to elevated HRG |
| Locus | 3q27, 3 |
| Discovery year | 1989-05-25 |
| Entrez gene record | 3273 |
| Pubmed identfication | 1678514 |
| RefSeq identity | NM_000412 |
| Classification | Cystatins, type 4 |
| Havana BLAST/BLAT | OTTHUMG00000156578 |
| Locus Specific Databases | LRG_601 |
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