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HSF1 Antibody, FITC Conjugated

HSF1 Antibody, FITC Conjugated size: 0.1ml 333

Price 333
Size 0.1ml
Long name FITC Conjugated, HSF1 Polyclonal Antibody
CategoryConjugated Primary Antibodies
ConjugationAnti-FITC Antibody
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenHSF1
SpecificityThis is a highly specific antibody against HSF1
ModificationUnmodified
Modification siteNone
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
Immunogen_range360-410/529
Subcellular location Nucleus, Cytoplasm
SourceKLH conjugated synthetic peptide derived from human HSF1
Gene ID number3297
Swiss ProtQ00613
Tested applicationsIF(IHC-P)
Recommended dilutionsIF(IHC-P)(1:50-200)
Crossreactivity Mouse, Rat, Human
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background HSF is unable to bind to the HSE unless the cells are heat shocked, In higher eukaryotes, DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation Emission494nm/518nm
Synonyms HSTF1
Properties Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targetHSF1 Conjugated
Short name FITC Conjugated, HSF1 Antibody
Technique Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelFITC
Alternative name fluorecein coupled, heat shock transcription factor 1 (Antibody to)
Alternative techniqueantibodies
Alternative to gene target DNA-templated and biological process this GO :0006355 and regulation of transcription, DNA-templated and biological process this GO :0006468 and protein phosphorylation and biological process this GO :0006952 and defense response and biological process this GO :0007143 and female meiotic division and biological process this GO :0007283 and spermatogenesis and biological process this GO :0008285 and negative regulation of cell proliferation and biological process this GO :0009408 and response to heat and biological process this GO :0032496 and response to lipopolysaccharide and biological process this GO :0032720 and negative regulation of tumor necrosis factor production and biological process this GO :0034605 and cellular response to heat and biological process this GO :0040018 and positive regulation of multicellular organism growth and biological process this GO :0043234 and protein complex and cellular component this GO :0043565 and sequence-specific DNA binding and molecular function this GO :0045087 and innate immune response and biological process this GO :0045120 and pronucleus and cellular component this GO :0045944 and positive regulation of transcription from RNA polymerase II promoter and biological process this GO :0060136 and embryonic process involved in female pregnancy and biological process this GO :0090231 and regulation of spindle checkpoint and biological process, HSF1 and IDBG-40498 and ENSG00000185122 and 3297, HSF1 and IDBG-643592 and ENSBTAG00000020751 and 506235, HSTF1, Hsf1 and IDBG-155311 and ENSMUSG00000022556 and 15499, nuclei, sequence-specific DNA binding, this GO :0000122 and negative regulation of transcription from RNA polymerase II promoter and biological process this GO :0000978 and RNA polymerase II core promoter proximal region sequence-specific DNA binding and molecular function this GO :0001078 and RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription and molecular function this GO :0001162 and RNA polymerase II intronic transcription regulatory region sequence-specific DNA binding and molecular function this GO :0001701 and in utero embryonic development and biological process this GO :0001892 and embryonic placenta development and biological process this GO :0003677 and DNA binding and molecular function this GO :0003682 and chromatin binding and molecular function this GO :0003700 and sequence-specific DNA binding transcription factor activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0006351 and transcription, this GO :0000978: RNA polymerase II core promoter proximal region sequence-specific DNA binding, this GO :0000978: RNA polymerase II core promoter proximal region sequence-specific DNA binding and also this GO :0001078: RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription and also this GO :0001162: RNA polymerase II intronic transcription regulatory region sequence-specific DNA binding and also this GO :0003677: DNA binding and also this GO :0003682: chromatin binding and also this GO :0003700: sequence-specific DNA binding transcription factor activity and also this GO :0005515: protein binding and also this GO :0043565: sequence-specific DNA binding, this GO :0001078: RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription, this GO :0001162: RNA polymerase II intronic transcription regulatory region sequence-specific DNA binding, this GO :0003677: DNA binding, this GO :0003682: chromatin binding, this GO :0003700: sequence-specific DNA binding transcription factor activity, this GO :0005515: protein binding, this GO :0043565: sequence-specific DNA binding, heat shock transcription factor 1
Identity 5224
Gene HSF1
Long gene name heat shock transcription factor 1
Locus 8q24, 3
Discovery year 1994-09-12
GenBank acession M64673
Entrez gene record 3297
Pubmed identfication 1871105
RefSeq identity NM_005526
Havana BLAST/BLAT OTTHUMG00000174604

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