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HSJ1 Antibody, Biotin Conjugated

HSJ1 Antibody, Biotin Conjugated size: 0.1ml 333

Price 333
Size 0.1ml
Long name Biotin Conjugated, HSJ1 Polyclonal Antibody
CategoryConjugated Primary Antibodies
ConjugationBiotinylated
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenHSJ1
SpecificityThis is a highly specific antibody against HSJ1
ModificationUnmodified
Modification siteNone
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
SourceKLH conjugated synthetic peptide derived from human HSJ1/DNAJB2
Gene ID number3300
Tested applications IHC-P, WB
Recommended dilutions IHC-P(1:100-500), WB(1:100-1000)
Crossreactivity Mouse, Rat, Human
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background DnaJ heat shock induced proteins are from the bacterium Escherichia coli and are under the control of the htpR regulatory protein, DnaJ proteins are important mediators of proteolysis and are involved in the regulation of protein degradation, DnaJB2 (DnaJ homolog subfamily B member 2), The DnaJ proteins play a critical role in the HSP 70 chaperone machine by interacting with HSP 70 to stimulate ATP hydrolysis, The presence of the J domain defines a protein as a member of the DnaJ family, The proteins contain cysteine rich regions that are composed of zinc fingers that form a peptide binding domain responsible for the chaperone function, Two isoforms are produced due to alternative splicing, also known as HSJ1 or HSPF3, exocytosis and endocytosis, is expressed almost exclusively in the brain, with the highest levels in the frontal cortex and hippocampus, The DnaJ family is one of the largest of all the chaperone families and has evolved with diverse cellular localization and functions
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation EmissionN/A
Synonyms HSPF3 CMT2T
Properties Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targetHSJ1 Conjugated
Short name Conjugated, HSJ1 Antibody
Technique antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelBiotin
Alternative name biotinilated coupled, HSJ1 (Antibody to)
Alternative techniqueantibodies
Identity 5228
Gene DNAJB2
Long gene name DnaJ heat shock protein family (Hsp40) member B2
Synonyms gene HSJ1
Synonyms gene name DnaJ (Hsp40) homolog, member 2 , subfamily B
Locus 2q35
Discovery year 1997-08-15
Entrez gene record 3300
Pubmed identfication 1599432 10516435
Classification DNAJ (HSP40) heat shock proteins
Havana BLAST/BLAT OTTHUMG00000133134

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