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IDH3A Antibody, FITC Conjugated

IDH3A Antibody, FITC Conjugated size: 0.1ml 333

Price 333
Size 0.1ml
Long name FITC Conjugated, IDH3A Polyclonal Antibody
CategoryConjugated Primary Antibodies
ConjugationAnti-FITC Antibody
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenIDH3A
SpecificityThis is a highly specific antibody against IDH3A
ModificationUnmodified
Modification siteNone
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
Immunogen_range310-360/366
Subcellular locationMitochondrion
SourceKLH conjugated synthetic peptide derived from human IDH3A
Gene ID number3419
Swiss ProtP50213
Tested applicationsIF(IHC-P)
Recommended dilutionsIF(IHC-P)(1:50-200)
Crossreactivity Mouse, Rat, Human
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background Each isozyme is a heterotetramer that is composed of two alpha subunits, Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, IDH3A is the alpha subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase, NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle, These enzymes belong to two distinct subclasses, and one gamma subunit, and two NADP(+)-dependent isocitrate dehydrogenases, one beta subunit, one of which is mitochondrial and the other predominantly cytosolic, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+), which localize to the mitochondrial matrix, Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation Emission494nm/518nm
Properties Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targetIDH3A Conjugated
Short name FITC Conjugated, IDH3A Antibody
Technique Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelFITC
Alternative name fluorecein coupled, IDH3A (Antibody to)
Alternative techniqueantibodies
Identity 5384
Gene IDH3A
Long gene name isocitrate dehydrogenase 3 (NAD(+)) alpha
Synonyms gene name isocitrate dehydrogenase 3 (NAD+) alpha
Synonyms name H-IDH alpha isocitric dehydrogenase isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial NAD+-specific ICDH NAD(H)-specific isocitrate dehydrogenase alpha subunit isocitrate dehydrogenase (NAD+) alpha chain
Locus 15q25, 1
Discovery year 1995-11-02
Entrez gene record 3419
Pubmed identfication 8833160
RefSeq identity NM_005530
Havana BLAST/BLAT OTTHUMG00000143732

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