| Antigen detectable with this ELISA kit | Insulin Like Growth Factor 2 (IGF2) |
| Species reactivity | Bovine (Cattle), Bos taurus |
| Range of detection | 1000pg/mL, 125pg/mL, 2000pg/mL, 250pg/mL, 4000pg/mL, 500pg/mL, 125-8000pg/mL The standard curve concentrations used for the ELISA&rsquo, s were 8000pg/mL |
| Sensitivity | The minimum detectable dose of this kit is typically less than 55pg/mL |
| Application | ELISA |
| Sample types tested for this ELISA kit | plasma and other biological fluids, Serum |
| Time for performing t hourse test | 4, 5 hoursours |
| Test principle | 10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Insulin Like Growth Factor 2 (IGF2), The concentration of Insulin Like Growth Factor 2 (IGF2) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Insulin Like Growth Factor 2 (IGF2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Insulin Like Growth Factor 2 (IGF2), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling | Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory | Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties | E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test | ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target | Insulin Like Growth Factor 2 (IGF2) |
| Short name | Insulin Like Growth Factor 2 (IGF2) ELISA kit |
| Technique | &hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host | antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name | Insulin Like Growth Factor 2 (insulin-like growth factor 2 (somatomedin A)) Enzyme-linked immunosorbent assay reagent |
| Alternative technique | elisas |
| Alternative to gene target | C11orf43 and IGF-II and PP9974, DNA-templated and biological process this GO :0007275 and multicellular organismal development and biological process this GO :0007565 and female pregnancy and biological process this GO :0007596 and blood coagulation and biological process this GO :0007613 and memory and biological process this GO :0008083 and growth factor activity and molecular function this GO :0008284 and positive regulation of cell proliferation and biological process this GO :0008286 and insulin receptor signaling pathway and biological process this GO :0009314 and response to radiation and biological process this GO :0009887 and organ morphogenesis and biological process this GO :0014070 and response to organic cyclic compound and biological process this GO :0030168 and platelet activation and biological process this GO :0030546 and receptor activator activity and molecular function this GO :0031017 and exocrine pancreas development and biological process this GO :0031093 and platelet alpha granule lumen and cellular component this GO :0031667 and response to nutrient levels and biological process this GO :0032355 and response to estradiol and biological process this GO :0035094 and response to nicotine and biological process this GO :0038028 and insulin receptor signaling pathway via phosphatidylinositol 3-kinase and biological process this GO :0042060 and wound healing and biological process this GO :0042104 and positive regulation of activated T cell proliferation and biological process this GO :0042493 and response to drug and biological process this GO :0043085 and positive regulation of catalytic activity and biological process this GO :0043410 and positive regulation of MAPK cascade and biological process this GO :0043539 and protein serine/threonine kinase activator activity and molecular function this GO :0044267 and cellular protein metabolic process and biological process this GO :0045471 and response to ethanol and biological process this GO :0045725 and positive regulation of glycogen biosynthetic process and biological process this GO :0045840 and positive regulation of mitosis and biological process this GO :0045944 and positive regulation of transcription from RNA polymerase II promoter and biological process this GO :0046628 and positive regulation of insulin receptor signaling pathway and biological process this GO :0050731 and positive regulation of peptidyl-tyrosine phosphorylation and biological process this GO :0051146 and striated muscle cell differentiation and biological process this GO :0051781 and positive regulation of cell division and biological process this GO :0051897 and positive regulation of protein kinase B signaling and biological process this GO :0070062 and extracellular vesicular exosome and cellular component this GO :0071260 and cellular response to mechanical stimulus and biological process this GO :0071902 and positive regulation of protein serine/threonine kinase activity and biological process this GO :0090031 and positive regulation of steroid hormone biosynthetic process and biological process this GO :2000273 and positive regulation of receptor activity and biological process this GO :2000467 and positive regulation of glycogen (starch) synthase activity and biological process, Extracellular, IGF2 and IDBG-20829 and ENSG00000167244 and 3481, IGF2 and IDBG-645244 and ENSBTAG00000013066 and 281240, Igf2 and IDBG-212623 and ENSMUSG00000048583 and 16002, protein serine/threonine kinase activator activity, this GO :0001501 and skeletal system development and biological process this GO :0001649 and osteoblast differentiation and biological process this GO :0001934 and positive regulation of protein phosphorylation and biological process this GO :0002576 and platelet degranulation and biological process this GO :0005158 and insulin receptor binding and molecular function this GO :0005159 and insulin-like growth factor receptor binding and molecular function this GO :0005179 and hormone activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005576 and extracellular region and cellular component this GO :0005615 and extracellular space and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0006006 and glucose metabolic process and biological process this GO :0006349 and regulation of gene expression by genetic imprinting and biological process this GO :0006355 and regulation of transcription, this GO :0005158: insulin receptor binding, this GO :0005158: insulin receptor binding and also this GO :0005159: insulin-like growth factor receptor binding and also this GO :0005179: hormone activity and also this GO :0005515: protein binding and also this GO :0008083: growth factor activity and also this GO :0030546: receptor activator activity and also this GO :0043539: protein serine/threonine kinase activator activity, this GO :0005159: insulin-like growth factor receptor binding, this GO :0005179: hormone activity, this GO :0005515: protein binding, this GO :0008083: growth factor activity, this GO :0030546: receptor activator activity, this GO :0043539: protein serine/threonine kinase activator activity, insulin-like growth factor 2 (somatomedin A) |
| Identity | 5466 |
| Gene | IGF2 |
| Long gene name | insulin like growth factor 2 |
| Synonyms gene | C11orf43 |
| Synonyms gene name | chromosome 11 open reading frame 43 insulin-like growth factor 2 |
| Synonyms | FLJ44734 IGF-II |
| Synonyms name | somatomedin A preptin |
| Locus | 11p15, 5 |
| Discovery year | 2001-06-22 |
| GenBank acession | M29645 AK025719 |
| Entrez gene record | 3481 |
| Pubmed identfication | 2450353 3167054 |
| RefSeq identity | NM_000612 |
| Havana BLAST/BLAT | OTTHUMG00000009395 |
| Locus Specific Databases | LOVD - Leiden Open Variation Database LRG_1031 |