| Antigen detectable with this ELISA kit | Intercellular Adhesion Molecule 4 (ICAM4) |
| Species reactivity | Homo sapiens (Human) |
| Range of detection | 0, 1, 25ng/mL, 3, 6, 12, 125ng/mL, 25ng/mL, 563ng/mL, 5ng/mL, 781-50ng/mL The standard curve concentrations used for the ELISA&rsquo, 781ng/mL, s were 50ng/mL |
| Sensitivity | 35ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application | ELISA |
| Sample types tested for this ELISA kit | cell lysates and other biological fluids, Tissue homogenates |
| Time for performing t hourse test | 4, 5 hoursours |
| Test principle | 10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Intercellular Adhesion Molecule 4 (ICAM4), The concentration of Intercellular Adhesion Molecule 4 (ICAM4) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Intercellular Adhesion Molecule 4 (ICAM4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Intercellular Adhesion Molecule 4 (ICAM4), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling | Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory | Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties | E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test | ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Description | present in lysates used as reference for ELISA quantification of these molecules and their subunits, Whole adhesion and interacting molecules are  |
| Gene target | Intercellular Adhesion Molecule 4 (ICAM4) |
| Short name | Intercellular Adhesion Molecule 4 (ICAM4) ELISA kit |
| Technique | &hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host | antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name | Intercellular Adhesion Molecule 4 (intercellular adhesion molecule 4 (Landsteiner-Wiener blood family)) Enzyme-linked immunosorbent assay reagent |
| Alternative technique | elisas |
| Alternative to gene target | CD242 and LW, Extracellular, ICAM4 and IDBG-26863 and ENSG00000105371 and 3386, ICAM4 and IDBG-643255 and ENSBTAG00000010314 and 514873, Icam4 and IDBG-138732 and ENSMUSG00000001014 and 78369, integrin binding, this GO :0005178 and integrin binding and molecular function this GO :0005576 and extracellular region and cellular component this GO :0005615 and extracellular space and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0016021 and integral component of membrane and cellular component this GO :0016337 and single organismal cell-cell adhesion and biological process this GO :0030198 and extracellular matrix organization and biological process this GO :0050776 and regulation of immune response and biological process, this GO :0005178: integrin binding, this GO :0005178: integrin binding, intercellular adhesion molecule 4 (Landsteiner-Wiener blood group) |
| Identity | 5347 |
| Gene | ICAM4 |
| Long gene name | intercellular adhesion molecule 4 (Landsteiner-Wiener blood group) |
| Synonyms gene | LW |
| Synonyms gene name | Landsteiner-Wiener blood group Landsteiner-Wiener blood group intercellular adhesion molecule 4 (LW blood group) , intercellular adhesion molecule 4 |
| Synonyms | CD242 |
| Locus | 19p13, 2 |
| Discovery year | 2001-06-22 |
| GenBank acession | X93093 |
| Entrez gene record | 3386 |
| Pubmed identfication | 8639917 6431896 |
| RefSeq identity | NM_001544 |
| Classification | CD molecules Immunoglobulin like domain containing Blood group antigens |
| Havana BLAST/BLAT | OTTHUMG00000180405 |
| Locus Specific Databases | Blood Group Antigen Mutation Database LRG_809 |