| Long name | FITC Conjugated, IRS1(Ser636 + Ser639) Antibody |
| Category | Conjugated Primary Antibodies |
| Conjugation | Anti-FITC Antibody |
| Host Organism | Rabbit (Oryctolagus cuniculus) |
| Target Antigen | IRS1 Ser636 + Ser639 |
| Specificity | Ser675 + Ser678 in Mouse, This phosphorylation site is homologous to Ser632 + ser635 in Mouse and Rat IRS1, This protein is known to be highly conserved in this sequences region, and Ser676 +Ser679 in Rat based on homology, this antibody may react with IRS2 when phosphorylated at Ser679 + Ser682 in Human , thus , This is a highly specific antibody against IRS1 Ser636 + Ser639 |
| Modification | Phosphorylation |
| Modification site | Ser636 + Ser639 |
| Clonality | Polyclonal |
| Clone | Polyclonal antibody |
| Concentration | 1ug per 1ul |
| Immunogen_range | 610-660/1242 |
| Subcellular location | Membrane bound, Nucleus, Cytoplasm |
| Source | KLH conjugated synthetic phosphopeptide derived from human IRS1 around the phosphorylation site of Ser636/639 [PM(p-S)PK(p-S)VS] |
| Gene ID number | 3667 |
| Swiss Prot | P35568 |
| Tested applications | IF(IHC-P) |
| Recommended dilutions | IF(IHC-P)(1:50-200) |
| Crossreactivity | Mouse, Rat, Human |
| Crossreactive species details | not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background | Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit (By similarity), When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2, May mediate the control of various cellular processes by insulin |
| Purification method | This antibody was purified via Protein A |
| Storage conditions | 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission | 494nm/518nm |
| Synonyms | HIRS-1 |
| Properties | Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target | IRS1(Ser636 + Ser639) Conjugated |
| Short name | FITC Conjugated, IRS1(Ser636 + Ser639) Antibody |
| Technique | Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label | FITC |
| Alternative name | fluorecein coupled, insulin receptor substrate 1(Ser636 and Ser639) (Antibody to) |
| Alternative technique | antibodies |
| Alternative to gene target | HIRS-1, IRS1 and IDBG-647770 and ENSBTAG00000021308 and 538598, IRS1 and IDBG-82407 and ENSG00000169047 and 3667, Irs1 and IDBG-173589 and ENSMUSG00000055980 and 16367, nuclei, phosphatidylinositol 3-kinase binding, this GO :0002053 and positive regulation of mesenchymal cell proliferation and biological process this GO :0004871 and signal transducer activity and molecular function this GO :0005068 and transmembrane receptor protein tyrosine kinase adaptor activity and molecular function this GO :0005080 and protein kinase C binding and molecular function this GO :0005158 and insulin receptor binding and molecular function this GO :0005159 and insulin-like growth factor receptor binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005829 and cytosol and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0005899 and insulin receptor complex and cellular component this GO :0005901 and caveola and cellular component this GO :0007165 and signal transduction and biological process this GO :0007173 and epidermal growth factor receptor signaling pathway and biological process this GO :0008284 and positive regulation of cell proliferation and biological process this GO :0008286 and insulin receptor signaling pathway and biological process this GO :0008543 and fibroblast growth factor receptor signaling pathway and biological process this GO :0010468 and regulation of gene expression and biological process this GO :0010907 and positive regulation of glucose metabolic process and biological process this GO :0014065 and phosphatidylinositol 3-kinase signaling and biological process this GO :0016042 and lipid catabolic process and biological process this GO :0019901 and protein kinase binding and molecular function this GO :0030335 and positive regulation of cell migration and biological process this GO :0030879 and mammary gland development and biological process this GO :0032000 and positive regulation of fatty acid beta-oxidation and biological process this GO :0032868 and response to insulin and biological process this GO :0032869 and cellular response to insulin stimulus and biological process this GO :0034504 and protein localization to nucleus and biological process this GO :0036064 and ciliary basal body and cellular component this GO :0038095 and Fc-epsilon receptor signaling pathway and biological process this GO :0042169 and SH2 domain binding and molecular function this GO :0042593 and glucose homeostasis and biological process this GO :0043231 and intracellular membrane-bounded organelle and cellular component this GO :0043434 and response to peptide hormone and biological process this GO :0043491 and protein kinase B signaling and biological process this GO :0043548 and phosphatidylinositol 3-kinase binding and molecular function this GO :0043552 and positive regulation of phosphatidylinositol 3-kinase activity and biological process this GO :0045087 and innate immune response and biological process this GO :0045725 and positive regulation of glycogen biosynthetic process and biological process this GO :0046326 and positive regulation of glucose import and biological process this GO :0046627 and negative regulation of insulin receptor signaling pathway and biological process this GO :0046628 and positive regulation of insulin receptor signaling pathway and biological process this GO :0046676 and negative regulation of insulin secretion and biological process this GO :0048009 and insulin-like growth factor receptor signaling pathway and biological process this GO :0048011 and neurotrophin TRK receptor signaling pathway and biological process this GO :0048015 and phosphatidylinositol-mediated signaling and biological process this GO :0060397 and JAK-STAT cascade involved in growth hormone signaling pathway and biological process this GO :2001275 and positive regulation of glucose import in response to insulin stimulus and biological process, this GO :0004871: signal transducer activity, this GO :0004871: signal transducer activity and also this GO :0005068: transmembrane receptor protein tyrosine kinase adaptor activity and also this GO :0005080: protein kinase C binding and also this GO :0005158: insulin receptor binding and also this GO :0005159: insulin-like growth factor receptor binding and also this GO :0005515: protein binding and also this GO :0019901: protein kinase binding and also this GO :0042169: SH2 domain binding and also this GO :0043548: phosphatidylinositol 3-kinase binding, this GO :0005068: transmembrane receptor protein tyrosine kinase adaptor activity, this GO :0005080: protein kinase C binding, this GO :0005158: insulin receptor binding, this GO :0005159: insulin-like growth factor receptor binding, this GO :0005515: protein binding, this GO :0019901: protein kinase binding, this GO :0042169: SH2 domain binding, this GO :0043548: phosphatidylinositol 3-kinase binding, insulin receptor substrate 1 |
| Identity | 6125 |
| Gene | IRS1 |
| Long gene name | insulin receptor substrate 1 |
| Locus | 2q36, 3 |
| Discovery year | 1992-08-24 |
| Entrez gene record | 3667 |
| Pubmed identfication | 1648180 |
| RefSeq identity | NM_005544 |
| Classification | Pleckstrin homology domain containing |
| Havana BLAST/BLAT | OTTHUMG00000133179 |
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