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JNK1+2+3 (T183+Y185) Antibody, FITC Conjugated

JNK1+2+3 (T183+Y185) Antibody, FITC Conjugated size: 0.1ml 350

Price 350
Size 0.1ml
Long name FITC Conjugated, JNK1+2+3 (T183+Y185) Polyclonal Antibody
CategoryConjugated Primary Antibodies
ConjugationAnti-FITC Antibody
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenJNK1+2+3 (THR183+TYR185)
SpecificityThis is a highly specific antibody against JNK1+2+3 (THR183+TYR185)
ModificationPhosphorylation
Modification siteTHR183+TYR185
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
Immunogen_range170-210/427
Subcellular location Nucleus, Cytoplasm
SourceKLH conjugated synthetic phosphopeptide derived from human JNK1 around the phosphorylation site of Thr183/Tyr185
Gene ID number5599
Swiss ProtP45983
Tested applicationsIF(IHC-P)
Recommended dilutionsIF(IHC-P)(1:50-200)
Crossreactivity Bovine, Dog, Mouse, Rat, Human
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background However, alpha-2, and beta-2 have a similar low level of binding to both c-Jun or ATF2, there is no correlation between binding and phosphorylation, whereas alpha-1, which is achieved at about the same efficiency by all isoforms, JNK1 isoforms display different binding patterns: beta-1 preferentially binds to c-Jun
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation Emission494nm/518nm
Synonyms JNK JNK1 SAPK1
Properties Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targetJNK1+2+3 (T183+Y185) Conjugated
Short name FITC Conjugated, JNK1+2+3 (T183+Y185) Antibody
Technique Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelFITC
Alternative name fluorecein coupled, JNK1+2+3 (T183+Y185) (Antibody to)
Alternative techniqueantibodies
Identity 6881
Gene MAPK8
Long gene name mitogen-activated protein kinase 8
Synonyms gene PRKM8
Synonyms name JUN N-terminal kinase
Locus 10q11, 22
Discovery year 1998-04-28
GenBank acession L26318
Entrez gene record 5599
Pubmed identfication 8137421 8654373
Classification Mitogen-activated protein kinases
Havana BLAST/BLAT OTTHUMG00000018172

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