Name : Kappa Light Chain Antibody, FITC Conjugated
Supplier : BIOSS PRIMARY CONJUGATED ANTIBODIES 1
Price :333
SKU : GEN3273116871
| Long name | FITC Conjugated, Kappa Light Chain Polyclonal Antibody |
| Category | Conjugated Primary Antibodies |
| Conjugation | Anti-FITC Antibody |
| Host Organism | Rabbit (Oryctolagus cuniculus) |
| Target Antigen | Kappa Light Chain |
| Specificity | This is a highly specific antibody against Kappa Light Chain |
| Modification | Unmodified |
| Modification site | None |
| Clonality | Polyclonal |
| Clone | Polyclonal antibody |
| Concentration | 1ug per 1ul |
| Source | Purified human Kappa light chain |
| Gene ID number | 10520 |
| Tested applications | IF(IHC-P) |
| Recommended dilutions | IF(IHC-P)(1:50-200) |
| Crossreactivity | Human |
| Crossreactive species details | not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background | Ala 45 and Leu 83, Ala 45 and Val 83, Antigens and immunoglobulins react to confer immunity to individuals, Both the heavy chains and the light chains are divided into constant and variable regions, IgA, IgA may also form polymers, IgD, IgG is divided into four subclasses, IgM, IgM is a pen tamer, Immunoglobulins have similar structures of two identical heavy chains and two identical light chains, In serum IgA and IgG are monomers with a single 4 polypeptide unit, Kappa light chain antibody can be used for the identification of leukemias, Kappa light chain contains one immunoglobulin like domain, The EU sequence has the INV allotypic marker, The ROY sequence has the INV allotypic marker, The amino acid sequence of the heavy chain determines the class of an immunoglobulin, The constant regions have the same amino acid sequences between all the immunoglobulin classes, The five types of immunoglobulin heavy chains are known as: IgG, The variable regions have approximately 110 amino acids with high sequence variability, and IgA is divided into two subclasses, and IgE, plasmacytomas and certain non Hodgkin's lymphomas, while, Immunoglobulins belong to a group of related glyco proteins which make up 20% of serum proteins |
| Purification method | This antibody was purified via Protein A |
| Storage conditions | 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission | 494nm/518nm |
| Synonyms | HCAK1, IGKC, Ig kappa chain C region, Immunoglobulin kappa constant, Immunoglobulin kappa constant region, Immunoglobulin kappa light chain, Kappa 1 immunoglobulin light chain, Km, MGC111575, MGC62011, MGC72072, MGC88770, MGC88771, MGC88809, HCAK 1 |
| Properties | Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target | Kappa Light Chain Conjugated |
| Short name | FITC Conjugated, Kappa Light Chain Antibody |
| Technique | Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label | FITC |
| Alternative name | fluorecein coupled, Kappa Light epitope (Antibody to) |
| Alternative technique | antibodies |