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LC3 Antibody, Biotin Conjugated

LC3 Antibody, Biotin Conjugated size: 0.1ml 333

Supplier BIOSS PRIMARY CONJUGATED ANTIBODIES 1
Price 333
Size 0.1ml
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
Long name Biotin Conjugated, LC3 Polyclonal Antibody
CategoryConjugated Primary Antibodies
ConjugationBiotinylated
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenLC3
SpecificityThis is a highly specific antibody against LC3
ModificationUnmodified
Modification siteNone
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
Immunogen_range27-59/121
SourceKLH conjugated synthetic peptide derived from human LC3
Gene ID number 81631, 84557
Swiss Prot Q9GZQ8, Q9H492
Tested applications IHC-P, WB
Recommended dilutions IHC-P(1:100-500), WB(1:100-1000)
Crossreactivity Mouse, Rat, Human
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production, Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway, Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation, Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes)
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation EmissionN/A
Synonyms MAP1BLC3 MAP1ALC3 LC3 LC3A ATG8E
Properties Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targetLC3 Conjugated
Short name Conjugated, LC3 Antibody
Technique antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelBiotin
Alternative name biotinilated coupled, LC3 (Antibody to)
Alternative techniqueantibodies
Identity 6838
Gene MAP1LC3A
Long gene name microtubule associated protein 1 light chain 3 alpha
Locus 20q11, 22
Discovery year 2000-06-29
Entrez gene record 84557
Pubmed identfication 8833088 17580304
RefSeq identity NM_181509
Classification Autophagy related
Havana BLAST/BLAT OTTHUMG00000032306

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