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LC3 Antibody, FITC Conjugated

LC3 Antibody, FITC Conjugated size: 0.1ml 333

Price 333
Size 0.1ml
Long name FITC Conjugated, LC3 Polyclonal Antibody
CategoryConjugated Primary Antibodies
ConjugationAnti-FITC Antibody
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenLC3
SpecificityThis is a highly specific antibody against LC3
ModificationUnmodified
Modification siteNone
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
Immunogen_range27-59/121
SourceKLH conjugated synthetic peptide derived from human LC3
Gene ID number 81631, 84557
Swiss Prot Q9GZQ8, Q9H492
Tested applicationsIF(IHC-P)
Recommended dilutionsIF(IHC-P)(1:50-200)
Crossreactivity Mouse, Rat, Human
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production, Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway, Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation, Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes)
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation Emission494nm/518nm
Synonyms MAP1BLC3 MAP1ALC3 LC3 LC3A ATG8E
Properties Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targetLC3 Conjugated
Short name FITC Conjugated, LC3 Antibody
Technique Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelFITC
Alternative name fluorecein coupled, LC3 (Antibody to)
Alternative techniqueantibodies
Identity 6838
Gene MAP1LC3A
Long gene name microtubule associated protein 1 light chain 3 alpha
Locus 20q11, 22
Discovery year 2000-06-29
Entrez gene record 84557
Pubmed identfication 8833088 17580304
RefSeq identity NM_181509
Classification Autophagy related
Havana BLAST/BLAT OTTHUMG00000032306

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