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Lin-28 Antibody, FITC Conjugated

Lin-28 Antibody, FITC Conjugated size: 0.1ml 333

Supplier BIOSS PRIMARY CONJUGATED ANTIBODIES 1
Price 333
Size 0.1ml
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
Long name FITC Conjugated, Lin-28 Polyclonal Antibody
CategoryConjugated Primary Antibodies
ConjugationAnti-FITC Antibody
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenLin-28
SpecificityThis is a highly specific antibody against Lin-28
ModificationUnmodified
Modification siteNone
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
Immunogen_range70-120/250
Subcellular location Nucleus, Cytoplasm
SourceKLH conjugated synthetic peptide derived from human Lin-28B
Gene ID number 79727, 389421
Swiss ProtQ6ZN17, Q9H9Z2
Tested applicationsIF(IHC-P)
Recommended dilutionsIF(IHC-P)(1:50-200)
Crossreactivity Mouse, Rat, Human
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, Isoform 1, Isoform 2 has no effect on cell growth, Mediates MYC-mediated let-7 repression, Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7, Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation, a miRNA precursor, leading to the terminal uridylation of pre-let-7, leading to their terminal uridylation and subsequent degradation, stimulates growth of the breast adenocarcinoma cell line MCF-7, when overexpressed, Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7)
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation Emission494nm/518nm
Synonyms CSDD 2, CSDD2, FLJ16517, LIN28B, LN28B_HUMAN, Lin 28, Lin 28 homolog B C, Lin-28A, Lin-28B, Protein lin-28 homolog A, Protein lin-28 homolog B, Zinc finger CCHC domain-containing protein 1, elegans, 2, Lin28B
Properties Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targetLin-28 Conjugated
Short name FITC Conjugated, Lin-28 Antibody
Technique Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelFITC
Alternative name fluorecein coupled, Lin-28 (Antibody to)
Alternative techniqueantibodies

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