| Long name | FITC Conjugated, LLGL1 + LLGL2(Ser650+Ser654) Antibody |
| Category | Conjugated Primary Antibodies |
| Conjugation | Anti-FITC Antibody |
| Host Organism | Rabbit (Oryctolagus cuniculus) |
| Target Antigen | LLGL1 + LLGL2 Ser650+Ser654 |
| Specificity | This is a highly specific antibody against LLGL1 + LLGL2 Ser650+Ser654 |
| Modification | Phosphorylation |
| Modification site | Ser650+Ser654 |
| Clonality | Polyclonal |
| Clone | Polyclonal antibody |
| Concentration | 1ug per 1ul |
| Source | KLH conjugated synthetic phosphopeptide derived from mouse LLGL1 around the phosphorylation site of Ser650+Ser654 |
| Gene ID number | 3996 |
| Tested applications | IF(IHC-P) |
| Recommended dilutions | IF(IHC-P)(1:50-200) |
| Crossreactivity | Mouse, Rat, Human |
| Crossreactive species details | not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background | In fly, In human, LLGL2 is a protein similar to lethal giant larvae of Drosophila, The gene for LLGL1 is located within the Smith-Magenis syndrome region on chromosome 17, The protein is part of a cytoskeletal network and is associated with nonmuscle myosin II heavy chain and a kinase that specifically phosphorylates this protein at serine residues, the protein's ability to localize cell fate determinants is regulated by the atypical protein kinase C (aPKC), this protein interacts with aPKC-containing complexes and is cortically localized in mitotic cells, LLGL1 is a protein that is similar to a tumor suppressor in Drosophila |
| Purification method | This antibody was purified via Protein A |
| Storage conditions | 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission | 494nm/518nm |
| Synonyms | HGL Hugl-2 |
| Properties | Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target | LLGL1 + LLGL2(Ser650+Ser654) Conjugated |
| Short name | FITC Conjugated, LLGL1 + LLGL2(Ser650+Ser654) Antibody |
| Technique | Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label | FITC |
| Alternative name | fluorecein coupled, lethal giant larvae homolog 1 (Drosophila) and lethal giant larvae homolog 2 (Drosophila)(Ser650+Ser654) (Antibody to) |
| Alternative technique | antibodies |
| Alternative to gene target | BT, Cytoplasm, Cytoplasm, DLG4 and HUGL and HUGL-1 and HUGL1 and LLGL, HGL and LGL2, LLGL1 and IDBG-33645 and ENSG00000131899 and 3996, LLGL1 and IDBG-638036 and ENSBTAG00000004635 and 781865, LLGL2 and IDBG-68503 and ENSG00000073350 and 3993, Llgl1 and IDBG-181336 and ENSMUSG00000020536 and 16897, Llgl2 and IDBG-214360 and ENSMUSG00000020782 and 217325, PDZ domain binding, lethal giant larvae homolog 2 (Drosophila), protein kinase binding, this GO :0000137 and this GO lgi cis cisterna and cellular component this GO :0000139 and this GO lgi membrane and cellular component this GO :0005097 and Rab GTPase activator activity and molecular function this GO :0005198 and structural molecule activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005737 and cytoplasm and cellular component this GO :0005856 and cytoskeleton and cellular component this GO :0006461 and protein complex assembly and biological process this GO :0006887 and exocytosis and biological process this GO :0006893 and this GO lgi to plasma membrane transport and biological process this GO :0007409 and axonogenesis and biological process this GO :0019901 and protein kinase binding and molecular function this GO :0030424 and axon and cellular component this GO :0030864 and cortical actin cytoskeleton and cellular component this GO :0030866 and cortical actin cytoskeleton organization and biological process this GO :0031901 and early endosome membrane and cellular component this GO :0032588 and trans- this GO lgi network membrane and cellular component this GO :0032851 and positive regulation of Rab GTPase activity and biological process this GO :0035090 and maintenance of apical/basal cell polarity and biological process this GO :0035748 and myelin sheath abaxonal region and cellular component, this GO :0005097: Rab GTPase activator activity, this GO :0005097: Rab GTPase activator activity and also this GO :0005198: structural molecule activity and also this GO :0005515: protein binding and also this GO :0019901: protein kinase binding, this GO :0005198: structural molecule activity, this GO :0005515 and protein binding and molecular function this GO :0005737 and cytoplasm and cellular component this GO :0006887 and exocytosis and biological process this GO :0007049 and cell cycle and biological process this GO :0030165 and PDZ domain binding and molecular function this GO :0032878 and regulation of establishment or maintenance of cell polarity and biological process this GO :0051301 and cell division and biological process, this GO :0005515: protein binding, this GO :0005515: protein binding, this GO :0005515: protein binding and also this GO :0030165: PDZ domain binding, this GO :0019901: protein kinase binding, this GO :0030165: PDZ domain binding, 52154 and IDBG-643198 and ENSBTAG00000020067 and 539545, lethal giant larvae homolog 1 (Drosophila) |
| Identity | 6629 |
| Gene | LLGL2 |
| Long gene name | LLGL2, scribble cell polarity complex component |
| Synonyms gene name | lethal giant larvae homolog 2 (Drosophila) lethal giant larvae homolog 2, scribble cell polarity complex component |
| Locus | 17q25, 1 |
| Discovery year | 1997-01-28 |
| GenBank acession | X87342 |
| Entrez gene record | 3993 |
| Pubmed identfication | 22580609 |
| RefSeq identity | NM_004524 |
| Classification | Scribble complex WD repeat domain containing |
| Havana BLAST/BLAT | OTTHUMG00000179685 |
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