| Antigen detectable with this ELISA kit | Low Density Lipoprotein Receptor Related Protein 5 (LRP5) |
| Species reactivity | Rattus norvegicus (Rat) |
| Range of detection | 0, 1, 25ng/mL, 3, 6, 12, 125ng/mL, 25ng/mL, 563ng/mL, 5ng/mL, 781-50ng/mL The standard curve concentrations used for the ELISA&rsquo, 781ng/mL, s were 50ng/mL |
| Sensitivity | 30ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application | ELISA |
| Sample types tested for this ELISA kit | cell lysates and other biological fluids, Tissue homogenates |
| Time for performing t hourse test | 4, 5 hoursours |
| Test principle | 10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Low Density Lipoprotein Receptor Related Protein 5 (LRP5), The concentration of Low Density Lipoprotein Receptor Related Protein 5 (LRP5) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Low Density Lipoprotein Receptor Related Protein 5 (LRP5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Low Density Lipoprotein Receptor Related Protein 5 (LRP5), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling | Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory | Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties | E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test | ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target | Lipoprotein Receptor Related Protein 5 (LRP5) |
| Short name | Low Density Lipoprotein Receptor Protein 5 (LRP5) ELISA kit |
| Technique | &hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host | antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name | Low Density Lipoprotein Receptor Related Protein 5 (low density lipoprotein receptor-related protein 5) Enzyme-linked immunosorbent assay reagent |
| Alternative technique | elisas |
| Alternative to gene target | BMND1 and EVR1 and EVR4 and HBM and LR3 and LRP-5 and LRP7 and OPPG and OPS and OPTA1 and VBCH2, DNA-templated and biological process this GO :0045944 and positive regulation of transcription from RNA polymerase II promoter and biological process this GO :0046849 and bone remodeling and biological process this GO :0046850 and regulation of bone remodeling and biological process this GO :0048539 and bone marrow development and biological process this GO :0048596 and embryonic camera-type eye morphogenesis and biological process this GO :0051091 and positive regulation of sequence-specific DNA binding transcription factor activity and biological process this GO :0060033 and anatomical structure regression and biological process this GO :0060042 and retina morphogenesis in camera-type eye and biological process this GO :0060059 and embryonic retina morphogenesis in camera-type eye and biological process this GO :0060070 and canonical Wnt signaling pathway and biological process this GO :0060348 and bone development and biological process this GO :0060349 and bone morphogenesis and biological process this GO :0060444 and branching involved in mammary gland duct morphogenesis and biological process this GO :0060603 and mammary gland duct morphogenesis and biological process this GO :0060612 and adipose tissue development and biological process this GO :0060764 and cell-cell signaling involved in mammary gland development and biological process this GO :0060828 and regulation of canonical Wnt signaling pathway and biological process this GO :0061178 and regulation of insulin secretion involved in cellular response to glucose stimulus and biological process this GO :0061299 and retina vasculature morphogenesis in camera-type eye and biological process this GO :0061304 and retinal blood vessel morphogenesis and biological process this GO :0071901 and negative regulation of protein serine/threonine kinase activity and biological process this GO :1902262 and apoptotic process involved in patterning of blood vessels and biological process, LRP5 and IDBG-61383 and ENSG00000162337 and 4041, LRP5 and IDBG-644859 and ENSBTAG00000005903 and 534450, Lrp5 and IDBG-128306 and ENSMUSG00000024913 and 16973, Plasma membranes, Wnt-activated receptor activity, this GO :0001702 and gastrulation with mouth forming second and biological process this GO :0001944 and vasculature development and biological process this GO :0002053 and positive regulation of mesenchymal cell proliferation and biological process this GO :0002076 and osteoblast development and biological process this GO :0005515 and protein binding and molecular function this GO :0005739 and mitochondrion and cellular component this GO :0005783 and endoplasmic reticulum and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0006007 and glucose catabolic process and biological process this GO :0006897 and endocytosis and biological process this GO :0008203 and cholesterol metabolic process and biological process this GO :0008217 and regulation of blood pressure and biological process this GO :0008284 and positive regulation of cell proliferation and biological process this GO :0009952 and anterior/posterior pattern specification and biological process this GO :0015026 and coreceptor activity and molecular function this GO :0016021 and integral component of membrane and cellular component this GO :0016055 and Wnt signaling pathway and biological process this GO :0017147 and Wnt-protein binding and molecular function this GO :0030326 and embryonic limb morphogenesis and biological process this GO :0033690 and positive regulation of osteoblast proliferation and biological process this GO :0035019 and somatic stem cell maintenance and biological process this GO :0035108 and limb morphogenesis and biological process this GO :0035426 and extracellular matrix-cell signaling and biological process this GO :0042074 and cell migration involved in gastrulation and biological process this GO :0042632 and cholesterol homeostasis and biological process this GO :0042733 and embryonic digit morphogenesis and biological process this GO :0042813 and Wnt-activated receptor activity and molecular function this GO :0042981 and regulation of apoptotic process and biological process this GO :0043235 and receptor complex and cellular component this GO :0043434 and response to peptide hormone and biological process this GO :0044332 and Wnt signaling pathway involved in dorsal/ventral axis specification and biological process this GO :0045600 and positive regulation of fat cell differentiation and biological process this GO :0045668 and negative regulation of osteoblast differentiation and biological process this GO :0045840 and positive regulation of mitosis and biological process this GO :0045893 and positive regulation of transcription, this GO :0005515: protein binding, this GO :0005515: protein binding and also this GO :0015026: coreceptor activity and also this GO :0017147: Wnt-protein binding and also this GO :0042813: Wnt-activated receptor activity, this GO :0015026: coreceptor activity, this GO :0017147: Wnt-protein binding, this GO :0042813: Wnt-activated receptor activity, low density lipoprotein receptor-related protein 5 |
| Identity | 6697 |
| Gene | LRP5 |
| Long gene name | LDL receptor related protein 5 |
| Synonyms gene | LRP7 OPPG EVR1 |
| Synonyms gene name | osteoporosis pseudoglioma syndrome exudative vitreoretinopathy 1 low density lipoprotein receptor-related protein 5 |
| Synonyms | LR3 BMND1 HBM OPS OPTA1 VBCH2 EVR4 |
| Locus | 11q13, 2 |
| Discovery year | 1998-04-07 |
| GenBank acession | AF064548 |
| Entrez gene record | 4041 |
| Pubmed identfication | 9714764 10049586 |
| RefSeq identity | NM_002335 |
| Classification | Low density lipoprotein receptors |
| Havana BLAST/BLAT | OTTHUMG00000167570 |
| Locus Specific Databases | LOVD - Leiden Open Variation Database |