Filters

Macrophage Migration Inhibitory Factor (MIF) ELISA kit

Macrophage Migration Inhibitory Factor (MIF) ELISA kit size: 1 plate of 96 wells 745

Price 745
Size 1 plate of 96 wells
Antigen detectable with this ELISA kitMacrophage Migration Inhibitory Factor (MIF)
Species reactivityHomo sapiens (Human)
Range of detection 0, 0, 1, 10ng/mL, 2, 5ng/mL, 25ng/mL, 313-20ng/mL The standard curve concentrations used for the ELISA&rsquo, 313ng/mL, 5ng/mL, 625ng/mL, s were 20ng/mL
Sensitivity126ng/mL, The minimum detectable dose of this kit is typically less than 0
ApplicationELISA
Sample types tested for this ELISA kit cell culture supernates and other biological fluids, cell lysates, plasma, tissue homogenates, Serum
Time for performing t hourse test4, 5 hoursours
Test principle 10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Macrophage Migration Inhibitory Factor (MIF), The concentration of Macrophage Migration Inhibitory Factor (MIF) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Macrophage Migration Inhibitory Factor (MIF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Macrophage Migration Inhibitory Factor (MIF), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn
Storage and handling Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt
Advisory Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes
Properties E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
TestELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Gene targetMacrophage Migration Inhibitory Factor (MIF)
Short nameMacrophage Migration Inhibitory Factor (MIF) ELISA kit
Technique &hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit
Hostantigen assay ELISA kit 1 Kit (96 Wells)
Alternative nameMacrophage Migration Inhibitory Factor (macrophage migration inhibitory factor (glycosylation-inhibiting factor)) Enzyme-linked immunosorbent assay reagent
Alternative techniqueelisas
Alternative to gene target Extracellular, GIF and GLIF and MMIF, MIF and IDBG-401910 and ENSG00000240972 and 4282, MIF and IDBG-637094 and ENSBTAG00000007375 and 280858, Mif and IDBG-163956 and ENSMUSG00000033307 and 17319, phenylpyruvate tautomerase activity, signal transduction by p53 class mediator and biological process this GO :0030890 and positive regulation of B cell proliferation and biological process this GO :0031666 and positive regulation of lipopolysaccharide-mediated signaling pathway and biological process this GO :0032269 and negative regulation of cellular protein metabolic process and biological process this GO :0033033 and negative regulation of myeloid cell apoptotic process and biological process this GO :0033138 and positive regulation of peptidyl-serine phosphorylation and biological process this GO :0042056 and chemoattractant activity and molecular function this GO :0042127 and regulation of cell proliferation and biological process this GO :0042327 and positive regulation of phosphorylation and biological process this GO :0043030 and regulation of macrophage activation and biological process this GO :0043066 and negative regulation of apoptotic process and biological process this GO :0043406 and positive regulation of MAP kinase activity and biological process this GO :0043518 and negative regulation of DNA damage response, signal transduction by p53 class mediator and biological process this GO :0045087 and innate immune response and biological process this GO :0048146 and positive regulation of fibroblast proliferation and biological process this GO :0050178 and phenylpyruvate tautomerase activity and molecular function this GO :0050715 and positive regulation of cytokine secretion and biological process this GO :0050731 and positive regulation of peptidyl-tyrosine phosphorylation and biological process this GO :0050918 and positive chemotaxis and biological process this GO :0061078 and positive regulation of prostaglandin secretion involved in immune response and biological process this GO :0061081 and positive regulation of myeloid leukocyte cytokine production involved in immune response and biological process this GO :0070062 and extracellular vesicular exosome and cellular component this GO :0070207 and protein homotrimerization and biological process this GO :0070374 and positive regulation of ERK1 and ERK2 cascade and biological process this GO :0071157 and negative regulation of cell cycle arrest and biological process this GO :0090238 and positive regulation of arachidonic acid secretion and biological process this GO :0090344 and negative regulation of cell aging and biological process this GO :1902166 and negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator and biological process this GO :2000343 and positive regulation of chemokine (C-X-C motif) ligand 2 production and biological process, this GO :0001516 and prostaglandin biosynthetic process and biological process this GO :0001934 and positive regulation of protein phosphorylation and biological process this GO :0002906 and negative regulation of mature B cell apoptotic process and biological process this GO :0004167 and dopachrome isomerase activity and molecular function this GO :0005102 and receptor binding and molecular function this GO :0005125 and cytokine activity and molecular function this GO :0005126 and cytokine receptor binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005576 and extracellular region and cellular component this GO :0005615 and extracellular space and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0006954 and inflammatory response and biological process this GO :0007166 and cell surface receptor signaling pathway and biological process this GO :0007569 and cell aging and biological process this GO :0008283 and cell proliferation and biological process this GO :0009986 and cell surface and cellular component this GO :0010629 and negative regulation of gene expression and biological process this GO :0010739 and positive regulation of protein kinase A signaling and biological process this GO :0019752 and carboxylic acid metabolic process and biological process this GO :0030330 and DNA damage response, this GO :0004167: dopachrome isomerase activity, this GO :0004167: dopachrome isomerase activity and also this GO :0005102: receptor binding and also this GO :0005125: cytokine activity and also this GO :0005126: cytokine receptor binding and also this GO :0005515: protein binding and also this GO :0042056: chemoattractant activity and also this GO :0050178: phenylpyruvate tautomerase activity, this GO :0005102: receptor binding, this GO :0005125: cytokine activity, this GO :0005126: cytokine receptor binding, this GO :0005515: protein binding, this GO :0042056: chemoattractant activity, this GO :0050178: phenylpyruvate tautomerase activity, macrophage migration inhibitory factor (glycosylation-inhibiting factor)
Identity 7097
Gene MIF
Long gene name macrophage migration inhibitory factor (glycosylation-inhibiting factor)
Synonyms gene GLIF
Synonyms GIF
Locus 22q11, 23
Discovery year 1993-10-15
GenBank acession M25639
Entrez gene record 4282
Pubmed identfication 7558020 2552447
RefSeq identity NM_002415
Havana BLAST/BLAT OTTHUMG00000150773

Similar products

Subscribe to our Newsletter