| Long name | 1, 2) Monoclonal Antibody, MAPK3 (327CT18 |
| Also known as | 1, 2) Monoclonal Antibody, MAPK3 (327CT18 |
| Category | Primary Antibodies |
| Conjugation | Unconjugated |
| Target Antigen | MAPK3 |
| Specificity | This is a highly specific antibody against MAPK3 |
| Modification(s) | None |
| Modification site(s) | Unmodified antibody |
| Clonality | Monoclonal |
| Clone number | 1, 2, 327CT18 |
| Source | This MAPK3 monoclonal antibody is generated from mouse immunized with MAPK3 recombinant protein |
| Gene ID number | 5595 |
| Swiss Prot | P27361 |
| Tested Applications | WB |
| Recommended dilutions | WB(1:100~8000) |
| Cross reactivity | Mouse, Human |
| Cross reactive species details | not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Background of the target antigen | About 160 substrates have already been discovered for ERKs, BCL6, BTG2, CASP9, CTTN, DAPK1, Depending on the cellular context, ELK1, ERF, FOS, FRS2 or GRB10), GJA1, HSF4 or SPZ1), However, IER3, MAP2, MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade, MAPT, MCL1 or PPARG), Many of these substrates are localized in the nucleus, Moreover, PXN, SORBS3 or STMN1), The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, The substrates include transcription factors (such as ATF2, They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF, adhesion, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors, and seem to participate in the regulation of transcription upon stimulation, and those are responsible for processes such as translation, as well as in the fragmentation of the Golgi apparatus during mitosis, cytoskeletal elements (such as CANX, cytoskeletal rearrangements, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC), mitosis, mitosis and apoptosis, other substrates are found in the cytosol as well as in other cellular organelles, regulators of apoptosis (such as BAD, regulators of translation (such as EIF4EBP1) and a variety of other signaling-related molecules (like ARHGEF2, survival and differentiation through the regulation of transcription, the MAPK/ERK cascade is also involved in the regulation of the endosomal dynamics, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, translation, Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway |
| Purification method | 09% (W/V) sodium azide, Mouse monoclonal antibody supplied in crude ascites with 0 |
| Storage conditions | 50% glycerol and 0, Store at -20°, 09% sodium azide, C for up to 1 year, Keep the antibody in aqueous buffered solution containing 1% BSA |
| Synonym names | ERK-1, ERT2, Extracellular signal-regulated kinase 1, HS44KDAP, HUMKER1A, Insulin-stimulated MAP2 kinase, MAP kinase 3, MAP kinase isoform p44, MAPK 3, MAPK3, Microtubule-associated protein 2 kinase, Mitogen-activated protein kinase 3, P44ERK1, P44MAPK, PRKM3, p44-ERK1, p44-MAPK, ERK1 |
| Properties | C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24° |
| About | Each murine monoclonal anibody has his own affinity specific for the clone, Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes, Monoclonals of this antigen are available in different clones |
| Gene target | 1, 2) Monoclonal, MAPK3 (327CT18 |
| Short name | 1, 2) Monoclonal Antibody, MAPK3 (327CT18 |
| Technique | Monoclonals or monoclonal antibodies, antibodies against human proteins, antibodies for, Antibody |
| Alternative name | 1, 2) monoclonal (Antibody to), mitogen-activated protein kinase 3 (327CT18 |
| Alternative technique | antibodies |
| Alternative to gene target | DNA-templated and biological process this GO :0006360 and transcription from RNA polymerase I promoter and biological process this GO :0006361 and transcription initiation from RNA polymerase I promoter and biological process this GO :0006468 and protein phosphorylation and biological process this GO :0006915 and apoptotic process and biological process this GO :0006974 and cellular response to DNA damage stimulus and biological process this GO :0006975 and DNA damage induced protein phosphorylation and biological process this GO :0007049 and cell cycle and biological process this GO :0007173 and epidermal growth factor receptor signaling pathway and biological process this GO :0007264 and small GTPase mediated signal transduction and biological process this GO :0007265 and Ras protein signal transduction and biological process this GO :0007411 and axon guidance and biological process this GO :0007596 and blood coagulation and biological process this GO :0008286 and insulin receptor signaling pathway and biological process this GO :0008543 and fibroblast growth factor receptor signaling pathway and biological process this GO :0009887 and organ morphogenesis and biological process this GO :0010467 and gene expression and biological process this GO :0015630 and microtubule cytoskeleton and cellular component this GO :0016032 and viral process and biological process this GO :0016310 and phosphorylation and biological process this GO :0016772 and transferase activity, ERK-1 and ERK1 and ERT2 and HS44KDAP and HUMKER1A and p44-ERK1 and p44-MAPK and P44ERK1 and P44MAPK and PRKM3, MAPK3 and IDBG-25745 and ENSG00000102882 and 5595, MAPK3 and IDBG-639178 and ENSBTAG00000016156 and 531391, Mapk3 and IDBG-209781 and ENSMUSG00000063065 and 26417, nuclei, this GO :0000165 and MAPK cascade and biological process this GO :0000186 and activation of MAPKK activity and biological process this GO :0000187 and activation of MAPK activity and biological process this GO :0001784 and phosphotyrosine binding and molecular function this GO :0001934 and positive regulation of protein phosphorylation and biological process this GO :0002224 and toll-like receptor signaling pathway and biological process this GO :0002755 and MyD88-dependent toll-like receptor signaling pathway and biological process this GO :0002756 and MyD88-independent toll-like receptor signaling pathway and biological process this GO :0004672 and protein kinase activity and molecular function this GO :0004707 and MAP kinase activity and molecular function this GO :0004713 and protein tyrosine kinase activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005524 and ATP binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005654 and nucleoplasm and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005739 and mitochondrion and cellular component this GO :0005769 and early endosome and cellular component this GO :0005770 and late endosome and cellular component this GO :0005794 and this GO lgi apparatus and cellular component this GO :0005829 and cytosol and cellular component this GO :0005856 and cytoskeleton and cellular component this GO :0005901 and caveola and cellular component this GO :0005925 and focal adhesion and cellular component this GO :0006351 and transcription, this GO :0001784: phosphotyrosine binding, this GO :0001784: phosphotyrosine binding and also this GO :0004672: protein kinase activity and also this GO :0004707: MAP kinase activity and also this GO :0004713: protein tyrosine kinase activity and also this GO :0005515: protein binding and also this GO :0005524: ATP binding and also this GO :0016772: transferase activity, this GO :0004672: protein kinase activity, this GO :0004707: MAP kinase activity, this GO :0004713: protein tyrosine kinase activity, this GO :0005515: protein binding, this GO :0005524: ATP binding, this GO :0016772: transferase activity, this GO :0019902: phosphatase binding, transferase activity, transferring phosphorus-containing groups, transferring phosphorus-containing groups and also this GO :0019902: phosphatase binding, transferring phosphorus-containing groups and molecular function this GO :0019221 and cytokine-mediated signaling pathway and biological process this GO :0019233 and sensory perception of pain and biological process this GO :0019902 and phosphatase binding and molecular function this GO :0030168 and platelet activation and biological process this GO :0030509 and BMP signaling pathway and biological process this GO :0031143 and pseudopodium and cellular component this GO :0031663 and lipopolysaccharide-mediated signaling pathway and biological process this GO :0032496 and response to lipopolysaccharide and biological process this GO :0032872 and regulation of stress-activated MAPK cascade and biological process this GO :0033129 and positive regulation of histone phosphorylation and biological process this GO :0034134 and toll-like receptor 2 signaling pathway and biological process this GO :0034138 and toll-like receptor 3 signaling pathway and biological process this GO :0034142 and toll-like receptor 4 signaling pathway and biological process this GO :0034146 and toll-like receptor 5 signaling pathway and biological process this GO :0034162 and toll-like receptor 9 signaling pathway and biological process this GO :0034166 and toll-like receptor 10 signaling pathway and biological process this GO :0035066 and positive regulation of histone acetylation and biological process this GO :0035666 and TRIF-dependent toll-like receptor signaling pathway and biological process this GO :0038083 and peptidyl-tyrosine autophosphorylation and biological process this GO :0038095 and Fc-epsilon receptor signaling pathway and biological process this GO :0038096 and Fc-gamma receptor signaling pathway involved in pha this GO cytosis and biological process this GO :0038123 and toll-like receptor TLR1:TLR2 signaling pathway and biological process this GO :0038124 and toll-like receptor TLR6:TLR2 signaling pathway and biological process this GO :0043330 and response to exogenous dsRNA and biological process this GO :0045087 and innate immune response and biological process this GO :0045944 and positive regulation of transcription from RNA polymerase II promoter and biological process this GO :0048011 and neurotrophin TRK receptor signaling pathway and biological process this GO :0051090 and regulation of sequence-specific DNA binding transcription factor activity and biological process this GO :0051216 and cartilage development and biological process this GO :0051403 and stress-activated MAPK cascade and biological process this GO :0051493 and regulation of cytoskeleton organization and biological process this GO :0060397 and JAK-STAT cascade involved in growth hormone signaling pathway and biological process this GO :0070062 and extracellular vesicular exosome and cellular component this GO :0070374 and positive regulation of ERK1 and ERK2 cascade and biological process this GO :0070498 and interleukin-1-mediated signaling pathway and biological process this GO :0070849 and response to epidermal growth factor and biological process this GO :0071260 and cellular response to mechanical stimulus and biological process this GO :0072584 and caveolin-mediated endocytosis and biological process this GO :0090170 and regulation of this GO lgi inheritance and biological process this GO :2000641 and regulation of early endosome to late endosome transport and biological process this GO :2000657 and negative regulation of apolipoprotein binding and biological process, mitogen-activated protein kinase 3 |
| Identity | 6877 |
| Gene | MAPK3 |
| Long gene name | mitogen-activated protein kinase 3 |
| Synonyms gene | PRKM3 |
| Synonyms | ERK1 p44mapk p44erk1 |
| Locus | 16p11, 2 |
| Discovery year | 1993-11-05 |
| GenBank acession | M84490 |
| Entrez gene record | 5595 |
| Pubmed identfication | 9628824 |
| Classification | Mitogen-activated protein kinases |
| Havana BLAST/BLAT | OTTHUMG00000132149 |
Technique
Supplier
Species
Label
Iso Type
Host
Tissue