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MAPKAPK2 (Thr222) Antibody, FITC Conjugated

MAPKAPK2 (Thr222) Antibody, FITC Conjugated size: 0.1ml 350

Supplier BIOSS PRIMARY CONJUGATED ANTIBODIES 1
Price 350
Size 0.1ml
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
Long name FITC Conjugated, MAPKAPK2 (Thr222) Polyclonal Antibody
CategoryConjugated Primary Antibodies
ConjugationAnti-FITC Antibody
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenMAPKAPK2 Thr222
SpecificityThis is a highly specific antibody against MAPKAPK2 Thr222
ModificationPhosphorylation
Modification siteThr222
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
Immunogen_range192-230/400
Subcellular location Nucleus, Cytoplasm
SourceKLH conjugated synthetic phosphopeptide derived from human MAPKAPK2 around the phosphorylation site of Thr222
Gene ID number9261
Swiss ProtP49137
Tested applicationsIF(IHC-P)
Recommended dilutionsIF(IHC-P)(1:50-200)
Crossreactivity Bovine, Mouse, Rat, Human
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background Also involved in late G2/M checkpoint following DNA damage through a process of post-transcriptional mRNA stabilization: following DNA damage, CDC25B, CDC25C, DNA damage response and transcriptional regulation, ELAVL1, Following stress, HNRNPA, HNRNPA, HSF1, HSP27/HSPB1, Hyd-X-R-X(2)-S, Involved in inflammatory response by regulating tumor necrosis factor (TNF) and IL6 production post-transcriptionally: acts by phosphorylating AU-rich elements (AREs)-binding proteins ELAVL1, Involved in toll-like receptor signaling pathway (TLR) in dendritic cells: required for acute TLR-induced macropinocytosis by phosphorylating and activating RPS6KA3, KRT18, KRT2, LIMK1, LSP1, Mediates phosphorylation of HSP27/HSPB1 in response to stress, PABPC1, PABPC1 and TTP/ZFP36, PARN, PDE4A, Phosphorylates ALOX5, Phosphorylates serine in the peptide sequence, Phosphorylation of TTP/ZFP36, RCSD1, RPS6KA3, TAB3 and TTP/ZFP36, a major post-transcriptional regulator of TNF, cell cycle control, cell migration, chromatin remodeling, endocytosis, it is phosphorylated and activated by MAP kinase p38-alpha/MAPK14, leading to dissociate HSP27/HSPB1 from large small heat-shock protein (sHsps) oligomers and impair their chaperone activities and ability to protect against oxidative stress effectively, leading to phosphorylation of substrates, leading to regulate the stability and translation of TNF and IL6 mRNAs, leading to stabilize GADD45A mRNA, promotes its binding to 14-3-3 proteins and reduces its ARE mRNA affinity leading to inhibition of dependent degradation of ARE-containing transcript, relocalizes from nucleus to cytoplasm and phosphorylates HNRNPA and PARN, reorganization of the cytoskeleton, where Hyd is a large hydrophobic residue, Stress-activated serine/threonine-protein kinase involved in cytokines production
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation Emission494nm/518nm
Properties Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targetMAPKAPK2 (Thr222) Conjugated
Short name FITC Conjugated, MAPKAPK2 (Thr222) Antibody
Technique Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelFITC
Alternative name fluorecein coupled, MAPKAPK2 (Thr222) (Antibody to)
Alternative techniqueantibodies
Identity 6887
Gene MAPKAPK2
Long gene name mitogen-activated protein kinase-activated protein kinase 2
Locus 1q32, 1
Discovery year 1999-03-26
GenBank acession U12779
Entrez gene record 9261
Pubmed identfication 8179591 8280084
RefSeq identity NM_004759
Classification Mitogen-activated protein kinase-activated protein kinases
Havana BLAST/BLAT OTTHUMG00000036342

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