| Antigen detectable with this ELISA kit | Matrix Metalloproteinase 3 (MMP3) |
| Species reactivity | Bovine (Cattle), Bos taurus |
| Range of detection | 1000pg/mL, 125pg/mL, 250pg/mL, 31, 500pg/mL, 62, 25-2000pg/mL The standard curve concentrations used for the ELISA&rsquo, 25pg/mL, 31, 5pg/mL, s were 2000pg/mL |
| Sensitivity | 2pg/mL, The minimum detectable dose of this kit is typically less than 14 |
| Application | ELISA |
| Sample types tested for this ELISA kit | plasma and other biological fluids, Serum |
| Time for performing t hourse test | 4, 5 hoursours |
| Test principle | 10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Matrix Metalloproteinase 3 (MMP3), The concentration of Matrix Metalloproteinase 3 (MMP3) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Matrix Metalloproteinase 3 (MMP3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Matrix Metalloproteinase 3 (MMP3), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling | Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory | Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties | E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test | ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target | Matrix Metalloproteinase 3 (MMP3) |
| Short name | Matrix Metalloproteinase 3 (MMP3) ELISA kit |
| Technique | &hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host | antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name | progelatinase)) Enzyme-linked immunosorbent assay reagent, Matrix Metalloproteinase 3 (matrix metallopeptidase 3 (stromelysin 1 |
| Alternative technique | elisas |
| Alternative to gene target | BT, CHDS6 and MMP-3 and SL-1 and STMY and STMY1 and STR1, Extracellular, MMP3 and IDBG-69254 and ENSG00000149968 and 4314, Mmp3 and IDBG-132579 and ENSMUSG00000043613 and 17392, progelatinase), this GO :0004175 and endopeptidase activity and molecular function this GO :0004222 and metalloendopeptidase activity and molecular function this GO :0005509 and calcium ion binding and molecular function this GO :0005576 and extracellular region and cellular component this GO :0005578 and proteinaceous extracellular matrix and cellular component this GO :0005615 and extracellular space and cellular component this GO :0006508 and proteolysis and biological process this GO :0008237 and metallopeptidase activity and molecular function this GO :0008270 and zinc ion binding and molecular function this GO :0010727 and negative regulation of hydrogen peroxide metabolic process and biological process this GO :0022617 and extracellular matrix disassembly and biological process this GO :0030198 and extracellular matrix organization and biological process this GO :0030334 and regulation of cell migration and biological process this GO :0030574 and collagen catabolic process and biological process this GO :0031012 and extracellular matrix and cellular component this GO :0071230 and cellular response to amino acid stimulus and biological process, this GO :0004175: endopeptidase activity, this GO :0004175: endopeptidase activity and also this GO :0004222: metalloendopeptidase activity and also this GO :0005509: calcium ion binding and also this GO :0008237: metallopeptidase activity and also this GO :0008270: zinc ion binding, this GO :0004222: metalloendopeptidase activity, this GO :0005509: calcium ion binding, this GO :0008237: metallopeptidase activity, this GO :0008270: zinc ion binding, zinc ion binding, 18504 and IDBG-629038 and ENSBTAG00000037768 and 100848277, matrix metallopeptidase 3 (stromelysin 1 |
| Identity | 7173 |
| Gene | MMP3 |
| Long gene name | matrix metallopeptidase 3 |
| Synonyms gene | STMY1 STMY |
| Synonyms gene name | matrix metalloproteinase 3 (stromelysin 1, progelatinase) stromelysin 1 |
| Locus | 11q22, 2 |
| Discovery year | 2001-06-22 |
| GenBank acession | X05232 |
| Entrez gene record | 4314 |
| RefSeq identity | NM_002422 |
| Classification | Matrix metallopeptidases |
| Havana BLAST/BLAT | OTTHUMG00000048254 |