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MCM4/CDC21 Antibody, FITC Conjugated

MCM4/CDC21 Antibody, FITC Conjugated size: 0.1ml 333

Supplier BIOSS PRIMARY CONJUGATED ANTIBODIES 1
Price 333
Size 0.1ml
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
Long name FITC Conjugated, MCM4/CDC21 Polyclonal Antibody
CategoryConjugated Primary Antibodies
ConjugationAnti-FITC Antibody
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenMCM4/CDC21
SpecificityThis is a highly specific antibody against MCM4/CDC21
ModificationUnmodified
Modification siteNone
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
SourceKLH conjugated synthetic peptide derived from human MCM4
Tested applicationsIF(IHC-P)
Recommended dilutionsIF(IHC-P)(1:50-200)
Crossreactivity Mouse, Rat, Human
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background Cleavage of MCM3, Expression of MCM proteins increases during cell growth, MCM proteins are regulated by E2F transcription factors, MCM2/MCM4 complexes function as substrates for Cdc2/cyclin B in vitro, MCM3, MCM4 (Cdc21), MCM5 (Cdc46), MCM6 (Mis5) and MCM7 (Cdc47), The MCM proteins each contain an ATP-binding motif, and by protein kinases, are regulators of DNA replication that act to ensure replication occurs only once in the cell cycle, including MCM2, peaking at G1 to S phase, results in the inactivation of the MCM complex (composed of at least MCM proteins 2-6) during apoptosis, which can be prevented by caspase inhibitors, which induce MCM expression, which interact with MCM proteins to maintain the postreplicative state of the cell, which is predicted to mediate ATP-dependent opening of double-stranded DNA, The mini-chromosome maintenance (MCM) family of proteins
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation Emission494nm/518nm
Synonyms CDC54 hCdc21 P1-Cdc21 MGC33310
Properties Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targetMCM4/CDC21 Conjugated
Short name FITC Conjugated, MCM4/CDC21 Antibody
Technique Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelFITC
Alternative name fluorecein coupled, MCM4/CDC21 (Antibody to)
Alternative techniqueantibodies
Identity 6947
Gene MCM4
Long gene name minichromosome maintenance complex component 4
Synonyms gene CDC21
Synonyms gene name MCM4 minichromosome maintenance deficient 4 (S, cerevisiae)
Locus 8q11, 21
Discovery year 1994-12-13
Entrez gene record 4173
Pubmed identfication 7601140
RefSeq identity NM_005914
Classification MCM family
Havana BLAST/BLAT OTTHUMG00000164205

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