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MEK5(Ser142) Antibody, FITC Conjugated

MEK5(Ser142) Antibody, FITC Conjugated size: 0.1ml 350

Supplier BIOSS PRIMARY CONJUGATED ANTIBODIES 1
Price 350
Size 0.1ml
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
Long name FITC Conjugated, MEK5(Ser142) Polyclonal Antibody
CategoryConjugated Primary Antibodies
ConjugationAnti-FITC Antibody
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenMEK5 Ser142
SpecificityThis is a highly specific antibody against MEK5 Ser142
ModificationPhosphorylation
Modification siteSer142
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
SourceKLH conjugated synthetic phosphopeptide derived from human MEK5 around the phosphorylation site of Ser142
Gene ID number5607
Tested applicationsIF(IHC-P)
Recommended dilutionsIF(IHC-P)(1:50-200)
Crossreactivity Mouse, Rat, Human
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background (MAP kinase kinase family), It is activated by phosphorylation on Ser/Thr by MAP kinase kinases and interacts specifically with ERK5, It is not phosphorylated by RAFA, MEK5 is expressed in many adult tissues and is most abundant in heart and skeletal muscle, RAFB or RAFC and it may interact with GTPases such as CDC42, The signal cascade mediated by this kinase is involved in growth factor stimulated cell proliferation and muscle cell differentiation, This kinase itself can be phosphorylated and activated by MAP3K3/MEKK3, This kinase specifically interacts with and activates MAPK7/ERK5, and not with another MAP kinase like P38, as well as by atypical protein kinase C isoforms (aPKCs), MEK5 is a dual specificity protein kinase belonging to thr Ser/Thr protein kinase family
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation Emission494nm/518nm
Synonyms MEK5 MAPKK5 HsT17454
Properties Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targetMEK5(Ser142) Conjugated
Short name FITC Conjugated, MEK5(Ser142) Antibody
Technique Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelFITC
Alternative name fluorecein coupled, MEK5(Ser142) (Antibody to)
Alternative techniqueantibodies
Identity 6845
Gene MAP2K5
Long gene name mitogen-activated protein kinase kinase 5
Synonyms gene PRKMK5
Locus 15q23
Discovery year 1997-11-11
GenBank acession U25265
Entrez gene record 5607
Pubmed identfication 7759517
RefSeq identity NM_145162
Classification Mitogen-activated protein kinase kinases
Havana BLAST/BLAT OTTHUMG00000133264

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