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MHC class I Antibody, FITC Conjugated

MHC class I Antibody, FITC Conjugated size: 0.1ml 333

Supplier BIOSS PRIMARY CONJUGATED ANTIBODIES 1
Price 333
Size 0.1ml
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
Long name FITC Conjugated, MHC class I Polyclonal Antibody
CategoryConjugated Primary Antibodies
ConjugationAnti-FITC Antibody
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenMHC class I
SpecificityThis is a highly specific antibody against MHC class I
ModificationUnmodified
Modification siteNone
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
Immunogen_range60-105/181
Subcellular locationExtracellular
SourceKLH conjugated synthetic peptide derived from human MHC class I
Swiss ProtG0ZMJ8
Tested applications IF(IHC-P), FCM
Recommended dilutions IF(IHC-P)(1:50-200), FCM(1:20-100)
CrossreactivityHuman
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen, Exon 1 encodes the leader peptide, Hundreds of HLA-A alleles have been described, Jul 2008], Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule, The heavy chain is anchored in the membrane, The heavy chain is approximately 45 kDa and its gene contains 8 exons, They are expressed in nearly all cells, This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin), Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation, [provided by RefSeq, and exons 6 and 7 encode the cytoplasmic tail, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, HLA-A belongs to the HLA class I heavy chain paralogues
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation Emission494nm/518nm
Synonyms 1C04_HUMAN, A 9, Aw 24, Aw 68, CLASS I HISTOCOMPATIBILITY ANTIGEN, H2 K1, H2K, HLA A, HLA C, HLA class I histocompatibility antigen A 1 alpha chain, HLA class I histocompatibility antigen A 11 alpha chain, HLA class I histocompatibility antigen A 2 alpha chain, HLA class I histocompatibility antigen A 24 alpha chain, HLA class I histocompatibility antigen A 3 alpha chain, HLA class I histocompatibility antigen A 30 alpha chain, HLA class I histocompatibility antigen A 32 alpha chain, HLA class I histocompatibility antigen A 68 alpha chain, HLA-A, HLA-C, HLAA, MHC class I heavy chain H2 K, Major histocompatibility complex class I A, A 28
Properties Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targetMHC class I Conjugated
Short name FITC Conjugated, MHC class I Antibody
Technique Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelFITC
Alternative name fluorecein coupled, Major histocompabitility complex class I (Antibody to)
Alternative techniqueantibodies

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