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MLH1 Antibody, ALEXA FLUOR 350

MLH1 Antibody, ALEXA FLUOR 350 size: 100ul 332

Supplier BIOSS PRIMARY CONJUGATED ANTIBODIES
Price 332
Size 100ul
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
Long name ALEXA FLUOR 350 Conjugated, MLH1 Polyclonal Antibody
Also known asAnti-MLH1 PAb ALEXA FLUOR 350
CategoryConjugated Primary Antibodies
Conjugated with 350, ALEXA FLUOR®
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenMLH1
SpecificityThis is a highly specific antibody against MLH1
ModificationUnmodified
Modification SiteNone
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
Immunogen range520-570/756
Subcellular locationNucleus
SourceThis antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human MLH1
Gene ID Number4292
Swiss ProtP40692
Tested applicationsIF(IHC-P)
Recommended dilutionsIF(IHC-P)(1:50-200)
Crossreactivity Mouse, Rat, Human
Cross-reactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Background of the antigen Also implicated in DNA damage signaling, Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2, DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected, DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH6) binding to a dsDNA mismatch, Heterodimerizes with MLH3 to form MutL gamma which plays a role in meiosis, It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch, MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, a component of the post-replicative DNA mismatch repair system (MMR), a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR, then MutL alpha is recruited to the heteroduplex, Heterodimerizes with PMS2 to form MutL alpha
PurificationPurified by Protein A
Storage conditions 50% glycerol and 0, Keep refrigerated at 2 to 8 degrees Celcius for up to one year, 09% sodium azide, Store this antibody in aqueous buffered solution containing 1% BSA
Excitation emission343nm/442nm
Synonyms HNPCC FCC2 HNPCC2
Properties ALEXA FLUOR they should be stored frozen at - 24°, Alexa Fluor 350 conjugates can be used in multi-color flow cytometry with FACS's equipped with a second red laser or red diode, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies, C, C for long term storage and for short term at + 5°, For facs or microscopy Alexa 1 conjugate
Conjugation ALEXA FLUOR 350, Alexa Fluor
Gene targetMLH1
Short nameMLH1 Antibody
Technique antibodies against human proteins, antibodies for, Antibody
LabelALEXA FLUOR 350
Alternative name ALEXA FLUOR 350, coli) (Antibody to), colon cancer, nonpolyposis classification 2 (E, mutL homolog 1
Alternative techniqueantibodies
Alternative to gene target COCA2 and FCC2 and hMLH1 and HNPCC and HNPCC2, MLH1 and IDBG-24600 and ENSG00000076242 and 4292, MLH1 and IDBG-644253 and ENSBTAG00000016758 and 533652, Mlh1 and IDBG-202755 and ENSMUSG00000032498 and 17350, coli), colon cancer, nonpolyposis type 2 (E, nuclei, structure-specific DNA binding, this GO :0000289 and nuclear-transcribed mRNA poly(A) tail shortening and biological process this GO :0000712 and resolution of meiotic recombination intermediates and biological process this GO :0000793 and condensed chromosome and cellular component this GO :0000794 and condensed nuclear chromosome and cellular component this GO :0000795 and synaptonemal complex and cellular component this GO :0001673 and male germ cell nucleus and cellular component this GO :0002204 and somatic recombination of immunoglobulin genes involved in immune response and biological process this GO :0003697 and single-stranded DNA binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005524 and ATP binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005694 and chromosome and cellular component this GO :0005712 and chiasma and cellular component this GO :0006200 and ATP catabolic process and biological process this GO :0006281 and DNA repair and biological process this GO :0006298 and mismatch repair and biological process this GO :0006303 and double-strand break repair via nonhomolo this GO us end joining and biological process this GO :0006974 and cellular response to DNA damage stimulus and biological process this GO :0007060 and male meiosis chromosome segregation and biological process this GO :0007129 and synapsis and biological process this GO :0007131 and reciprocal meiotic recombination and biological process this GO :0007140 and male meiosis and biological process this GO :0007283 and spermatogenesis and biological process this GO :0008630 and intrinsic apoptotic signaling pathway in response to DNA damage and biological process this GO :0016020 and membrane and cellular component this GO :0016446 and somatic hypermutation of immunoglobulin genes and biological process this GO :0016447 and somatic recombination of immunoglobulin gene segments and biological process this GO :0016887 and ATPase activity and molecular function this GO :0030983 and mismatched DNA binding and molecular function this GO :0032137 and guanine/thymine mispair binding and molecular function this GO :0032300 and mismatch repair complex and cellular component this GO :0032389 and MutLalpha complex and cellular component this GO :0032407 and MutSalpha complex binding and molecular function this GO :0043060 and meiotic metaphase I plate congression and biological process this GO :0043566 and structure-specific DNA binding and molecular function this GO :0045132 and meiotic chromosome segregation and biological process this GO :0045190 and isotype switching and biological process this GO :0045950 and negative regulation of mitotic recombination and biological process this GO :0048477 and oogenesis and biological process this GO :0051257 and spindle midzone assembly involved in meiosis and biological process, this GO :0003697: single-stranded DNA binding, this GO :0003697: single-stranded DNA binding and also this GO :0005515: protein binding and also this GO :0005524: ATP binding and also this GO :0016887: ATPase activity and also this GO :0030983: mismatched DNA binding and also this GO :0032137: guanine/thymine mispair binding and also this GO :0032407: MutSalpha complex binding and also this GO :0043566: structure-specific DNA binding, this GO :0005515: protein binding, this GO :0005524: ATP binding, this GO :0016887: ATPase activity, this GO :0030983: mismatched DNA binding, this GO :0032137: guanine/thymine mispair binding, this GO :0032407: MutSalpha complex binding, this GO :0043566: structure-specific DNA binding, mutL homolog 1
Identity 7127
Gene MLH1
Long gene name mutL homolog 1
Synonyms gene COCA2
Synonyms gene name coli) , coli) homolog 1 (colon cancer, colon cancer, mutL (E, nonpolyposis type 2 (E, nonpolyposis type 2) mutL homolog 1
Locus 3p22, 2
Discovery year 1993-11-24
GenBank acession U07418
Entrez gene record 4292
Pubmed identfication 7903889
RefSeq identity NM_000249
Classification MutL homologs
Havana BLAST/BLAT OTTHUMG00000130797
Locus Specific Databases HNPCC Colon cancer gene variant databases MLH1 database at LOVD-China LOVD - Leiden Open Variation Database LRG_216 , Hereditary Non-Polyposis Colorectal Cancer

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