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Mouse Anti-CD22, conjugated to PE

Mouse Anti-CD22, conjugated to PE size: 2 ml 431

Supplier MBS MONO
Price 431
Size 2 ml
Also known asCD22
Other names B-cell receptor CD22, B-cell receptor CD22, B-lymphocyte cell adhesion molecule, B-lymphocyte cell adhesion molecule, BL-CAM, BL-CAM, CD22 antigen, CD22 molecule, CD_antigen: CD22, Sialic acid-binding Ig-like lectin 2, Siglec-2, T-cell surface antigen Leu-14, T-cell surface antigen Leu-14, partial, sialic acid binding Ig-like lectin 2, sialic acid-binding Ig-like lectin 2, CD22
CategoryAntibodies
SubcategoryMnoclonal antibodies
Gene nameCD22
Gene name synonimsN/A
Other gene names BL-CAM, CD22, SIGLEC-2, SIGLEC2, SIGLEC2, Siglec-2, CD22
ClonalityMonoclonal antibody
Immunoglobulin isotypeIgG1
CloneRFB4
Host organismMouse (Mus musculus)
Species reactivity Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby, Human (Homo sapiens)
Specificity and cross-reactivity 50 ul of leukocytes containing 10^7 cells/ml are stained with 20 ul mAb of various dilutions to obtain a titration curve and to identify the saturation point and detection threshold, In addition and to control lot-to-lot variation, In practice, Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited, The final concentration of the product is then adjusted to be at least 3-fold above the detection threshold, The sensitivity of RFB4 mAb is determined by staining well-defined blood samples from representative donors with serial-fold mAb dilutions to obtain a titration curve that allows relating the mAb concentration to the percentage of stained cells and geometric MFI (mean fluorescence intensity), This particular antibody might cross react with speacies outside of the listed ones, a mAb-concentration range is selected to include both the saturation point (i, the given lot is compared and adjusted to fluorescence standards with defined intensity, the mAb dilution expected to bind all epitopes on the target cell) and the detection threshold (i, the mAb dilution expected to represent the least amount of mAb needed to detect an identical percentage of cells), For this purpose, The CD22 mAb (clone RFB4) recognizes surface CD22 expressed by mature peripheral B-cells and cytoplasmatic CD22 expressed by precursor B-cells, e, e
Purification methodN/A
Form/Appearance 0, 1% BSA, Storage buffer: PBS pH 7, 05% NaN3, 2, Purified by Chromatography
ConcentrationN/A
Storage and shipping Keep the antibody at +4 degrees Celsius and do NOT freeze it, Please refer to the expiry date printed on the vial, The use of the reagent after the expiration date is not recommended, To enchance the antibody's stability sodium azide has been added, the antibody may be photosensitive, therefore avoid exposing the vial to direct bright light, the antibody contains monoclonal antibody specific reagents in optimal concentrations of affinity-purified antibody
Tested applications: Indirect Immunofluorescence (IF), Flow Cytometry (FC/FACS)
Test A/J, BALB/c, Mouse are mature after 40 days for females and 55 days for males, SCID while the CD-1 is outbred as strain, The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year, Transgenic, congenic and inbread strains are known for C57BL/6, knock-out, Mouse , mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab, or 
Latin nameMus musculus
Gene target conjugated PE, CD22
Short name conjugated PE, Mouse Anti-CD22
Technique mouses, Mouse
LabelPE
Species Mouses, Mouse
Alternative name coupled to peroxidase, Mouse antibody to-CD22 molecule
Alternative techniquemurine
Alternative to gene target CD22 and IDBG-44001 and ENSG00000012124 and 933, CD22 and IDBG-635610 and ENSBTAG00000011887 and 514582, Cd22 and IDBG-171574 and ENSMUSG00000030577 and 12483, Cell surfaces, SIGLEC-2 and SIGLEC2, protein binding, this GO :0005515 and protein binding and molecular function this GO :0005887 and integral component of plasma membrane and cellular component this GO :0007155 and cell adhesion and biological process this GO :0009897 and external side of plasma membrane and cellular component this GO :0045087 and innate immune response and biological process this GO :0070062 and extracellular vesicular exosome and cellular component, this GO :0005515: protein binding, this GO :0005515: protein binding, CD22 molecule
Identity 1643
Gene CD22
Long gene name CD22 molecule
Synonyms gene name CD22 antigen
Synonyms SIGLEC-2 SIGLEC2
Synonyms name sialic acid binding Ig-like lectin 2
Locus 19q13, 12
Discovery year 1991-06-06
GenBank acession X52785
Entrez gene record 933
Pubmed identfication 8496602 1691828
RefSeq identity NM_001771
Classification C2-set domain containing CD molecules Sialic acid binding Ig like lectins
Havana BLAST/BLAT OTTHUMG00000183298

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