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Mouse Anti-CD3 Antibody

Mouse Anti-CD3 Antibody size: 200ug 359

Supplier MBS MONO
Price 359
Size 200ug
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
Also known asCD3
Other names CD3gamma/delta, CD3gamma/delta, N/A, N/A, cd3
CategoryAntibodies
SubcategoryMnoclonal antibodies
Gene nameCD3
Gene name synonimsN/A
Other gene names N/A, N/A, cd3g, N/A
ClonalityMonoclonal antibody
Immunoglobulin isotypeIgG1
CloneUCHT1
Host organismMouse (Mus musculus)
Species reactivity Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby, Human (Homo sapiens)
Specificity and cross-reactivity 50 ul of leukocytes containing 107 cells/ml are stained with 20 ul mAb of various dilutions to obtain a titration curve and to identify the saturation point and detection threshold, In addition and to control lot-to-lot variation, Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited, The final concentration of the product is then adjusted to be at least 3-fold above the detection threshold, The sensitivity of UCHT1 mAb is determined by staining well-defined blood samples from representative donors with serial-fold mAb dilutions to obtain a titration curve that allows relating the mAb concentration to the percentage of stained cells and geometric MFI (mean fluorescence intensity), This particular antibody might cross react with speacies outside of the listed ones, the given lot is compared and adjusted to fluorescence standards with defined intensity, In practice, The CD3 mAb (clone UCHT1) recognizes cytoplasmic CD3 epsilon in precursor T-cells and cytoplasmic and surface CD3 epsilon in mature T-lymphocytes, The sensitivity of UCHT1 mAb is determined by staining well-defined blood samples from representative donors with serial-fold mAb dilutions to obtain a titration curve that allows relating the mAb concentration to the percentage of stained cells and geometric MFI (mean fluorescence intensity)
Purification methodN/A
Form/Appearance 0, 1% BSA, Storage buffer: PBS pH 7, 05% NaN3, 2, Purified by Chromatography
ConcentrationN/A
Storage and shipping Keep the antibody at +4 degrees Celsius and do NOT freeze it, Please refer to the expiry date printed on the vial, The use of the reagent after the expiration date is not recommended, To enchance the antibody's stability sodium azide has been added, the antibody may be photosensitive, therefore avoid exposing the vial to direct bright light, the antibody contains monoclonal antibody specific reagents in optimal concentrations of affinity-purified antibody
Tested applications: Indirect Immunofluorescence (IF), Flow Cytometry (FC/FACS)
PropertiesC, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by MBS mono they should be stored frozen at - 24°
Test A/J, BALB/c, Mouse are mature after 40 days for females and 55 days for males, SCID while the CD-1 is outbred as strain, The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year, Transgenic, congenic and inbread strains are known for C57BL/6, knock-out, Mouse , mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab, or 
Latin nameMus musculus
Gene targetCD3
Short nameMouse Anti-CD3 Antibody
Technique antibodies against human proteins, antibodies for, mouses, Antibody
Species Mouses, Mouse
Alternative nameMouse antibody to-CD3 (Antibody to)
Alternative techniqueantibodies
Identity 24219
Gene CD3EAP
Long gene name CD3e molecule associated protein
Synonyms gene name CD3e antigen, epsilon associated protein , epsilon polypeptide associated protein CD3e molecule
Synonyms ASE-1 CAST PAF49
Synonyms name CD3 epsilon associated protein antisense to ERCC 1
Locus 19q13, 32
Discovery year 2005-04-21
GenBank acession U86751
Entrez gene record 10849
Pubmed identfication 10373416 9426281 15226435
RefSeq identity NM_012099
Havana BLAST/BLAT OTTHUMG00000182120

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