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MOUSE Anti-HISTIDINE TAG:Biotin Antibody

MOUSE Anti-HISTIDINE TAG:Biotin Antibody size: 100ug 520

Supplier MBS MONO
Price 520
Size 100ug
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
Also known asHISTIDINE TAG
Other namesN/A
CategoryAntibodies
SubcategoryMnoclonal antibodies
Gene nameN/A
Gene name synonimsN/A
Other gene namesN/A
ClonalityMonoclonal
Immunoglobulin isotypeIgG1
Clone1, 10, AD1
Host organismMouse (Mus musculus)
Species reactivity Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby, Synthetic Peptide
Specificity and cross-reactivity In Western blotting of bacterial extracts the antibody has been shown not to cross-react with any endogenous products, It reacts against all HIS-tagged proteins so far tested, Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited, The antibody may be used to track the purification of proteins expressed in bacteria, This particular antibody might cross react with speacies outside of the listed ones, _x000D__x000D_This product is routinely tested in ELISA using histidine tagged ubiquitin and in Western blotting using HIS-tagged recombinant protein control, although some cross-reactivity may be seen with extracts of insect or mammalian cells, yeast or insect cells, HISTIDINE TAG This item recognises proteins containing the motif H-H-H-H-H-H and is therefore of value in detecting proteins containing histidine tags
Purification methodN/A
Form/AppearanceBiotin (Purified IgG conjugated to Biotin - liquid)
Concentration0 mg/ml, IgG concentration 1
Storage and shipping If there is a precipitate in the vial we recommend you to briefly microcentrifugate it prior to use, Repeated freeze - thaw cycles may denature the peptide chains of the antibody and therefore should be maximally avoided, Shelf Life: 18 months from date of dispatch, Storage in frost free freezers is not recommended, the antibody should be stored undiluted, Store the antibody at +4 degrees Celsius for short-term storage and at -20 degrees Celsius for long-term
Tested applications: Western Blot (WB), ELISA (EIA)
Properties Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by MBS mono they should be stored frozen at - 24°
ConjugationBiotinylated
Gene targetHISTIDINE TAG:
Short nameMOUSE Anti-HISTIDINE TAG: Antibody
Technique antibodies against human proteins, antibodies for, Antibody
LabelBiotin
Alternative nameMOUSE antibody to-HISTIDINE detection labelled:biotinilated (Antibody to)
Alternative techniqueantibodies

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